The AMSBIO Cultrex® 3D
Culture Cell Harvesting Kit is based on a non-enzymatic approach that prevents
biochemical degradation during processing and overcomes the problems associated
with extracting cells from 3D culture in BME or Laminin I. The quality of the
harvested cells is ideal for Western blotting and RNA and DNA analysis. In 3D
culture, cells are grown in a microenvironment of specialized proteins that
mimics in vivo conditions and allows the cells to organise into complex
structures rather than uniform monolayers.
This new kit provides an optimised and standardised solution for the isolation
and normalization of cell lysates from 3D Culture Matrix, BME or Laminin I for
subsequent biochemical analysis.
3D Cultures exhibit cellular behaviours and morphologies similar to those seen
in vivo; however, the adaptation of these models for studying biochemical
processes has been impeded by the challenge of separating intact or 3D grown
cells from extracellular proteins comprising the 3D matrix. Commonly,
proteases are employed to degrade these extracellular proteins; however,
proteases also degrade proteins on the cell surface, and protease activity may
carry over into lysate preparations. Non-enzymatic methodologies have also been
described for depolymerising extracellular matrix proteins, although the
implementation of these protocols remains problematic for some researchers.
For further information please contact AMSBIO on +44-1235-828200 /
+1-800-987-0985 or email
info@amsbio.com.
Founded in 1987, AMS Biotechnology (AMSBIO) is recognised as a leading
international provider of unique, innovative products & custom services for
life sciences research. The AMSBIO range includes over 23,000 polyclonal &
monoclonal antibodies, peptides, recombinant proteins, extracellular matrix,
molecular detection reagents, & tissue DNA, RNA, protein & microarray
products. Key research areas include: apoptosis, cell invasion & migration,
cell signaling, DNA damage, 3D culture, electrophoresis, glycobiology,
post-translational modification & stem cell biology.
