Thermo Fisher Scientific is pleased to announce
a new approach using high-performance anion-exchange (
HPAE) chromatography with pulsed amperometric
detection (PAD) for the determination of
carbohydrates in extracts from instant coffee
and green coffee beans. Application Note 280:
Carbohydrate in Coffee: AOAC Method 995.13 vs a New Fast
Ion Chromatography Method compares
two methods: the Association of Analytical Chemists (
AOAC) official method 995.13 and a fast method
using the
CarboPac TM SA10 column . The former
method has a longer run time (80 minutes) compared to the fast method (10
minutes). For certain sugars that may be difficult to resolve with the official
method, minor modifications are suggested. The fast method, proposed for
analyzing coffee carbohydrates, resolves 7 of the 11 coffee carbohydrates in 8
minutes and needs only the addition of deionized water for continuous
operation.
Both methods have high precision and acceptable
recovery for the carbohydrates in instant and green coffee extracts. In
addition,
disposable gold working electrodes provide
consistently high detector response for both methods, assuring greater
instrument-to-instrument and lab-to-lab reproducibility. In summary, both the
AOAC official method 995.13 and the fast method are sensitive, accurate,
reliable, and differ primarily in their total analysis time and peak
resolutions for coffee carbohydrate determinations.
This application note and many others can be
found at
www.thermoscientific.com/dionex
under the
Documents tab.
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