Thermo Fisher Scientific is pleased to announce
a new method that uses the Thermo Scientific
Dionex Corona CAD Charged Aerosol Detector to
provide a reliable alternative to low-wavelength
UV detection. Customer Application Note (CAN)
112:
Determination of
Ginsenosides in Panax ginseng by HPLC-CAD demonstrates that charged aerosol detection
does not overrespond to strong
chromophores, thereby reducing interferences.
Baseline slopes are not as pronounced or as variable, making peak area
determinations more reliable.
Asian ginseng
(Panax ginseng) traditionally
is used as a tonic to reduce the effects of stress, counteract fatigue, and
increase stamina. The main bioactive ingredients found in
Panax ginseng
and a related species,
Panax quinquefolius (American ginseng), are
triterpene saponins, commonly referred to as
ginsenosides. Most methods for the analysis of
ginsenosides use gradient elution reversed-phase high-performance liquid
chromatography (RP-HPLC) with low-wavelength UV detection (203-205 nm) because
the ginsenosides do not strongly absorb above 205 nm. This often results
in strongly sloping baselines that complicate integration, and interferences
from minor components that have stronger UV chromophores than the ginsenosides.
This customer application note and many others
can be found at
www.thermoscientific.com/dionex
under the
Documents tab.
