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Determination of Steviol Glycoside in Sweeteners
Thermo Fisher Scientific Inc. is pleased to announce an economical new method that uses high-performance liquid chromatography (HPLC) with charged aerosol and UV detections to determine steviol glycosides in consumer sweeteners. Application Note 293: Steviol Glycoside Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column demonstrates that only 3-9 mL of mobile phase/sample are required, compared to 25-60 mL/sample for the Joint FAO/WHO Commission on Food Additives ( JECFA) and U.S. Pharmacopeia (USP) methods, respectively. The reduction in mobile phase use saves both time and resources during mobile phase preparation and also reduces waste.
Using the Thermo Scientific Acclaim Trinity P1 column, the proposed method resolves the steviol glycosides of interest. Quantification of the two principal glycosides, stevioside and rebaudioside A, can be accomplished by both detection methods. Charged aerosol detection has the advantage of distinguishing additional components in the sample that are not UV absorbing, allowing for a flexible method to check sweetener purity.
This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
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