Thermo Fisher Scientific
Inc. is pleased to announce an economical new method that uses high-performance
liquid chromatography (
HPLC) with
charged aerosol and
UV detections to determine steviol glycosides
in consumer sweeteners. Application Note 293:
Steviol Glycoside Determination by HPLC with Charged
Aerosol and UV Detections Using the Acclaim Trinity P1 Column demonstrates that only 3-9 mL of mobile
phase/sample are required, compared to 25-60 mL/sample for the Joint FAO/WHO
Commission on Food Additives (
JECFA) and U.S. Pharmacopeia (
USP) methods, respectively. The reduction in
mobile phase use saves both time and resources during mobile phase preparation
and also reduces waste.
Using the Thermo Scientific Acclaim Trinity P1 column, the proposed method
resolves the steviol glycosides of interest. Quantification of the two
principal glycosides, stevioside and rebaudioside A, can be accomplished by
both detection methods. Charged aerosol detection has the advantage of
distinguishing additional components in the sample that are not UV absorbing,
allowing for a flexible method to check sweetener purity.
This application note and many others can be found at
www.thermoscientific.com/dionex under the
Documents tab.
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