Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

New Application Note from BMG LABTECH - "Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma Viability"

Quantitative, High- Throughput, Fluorescent-Based Bioassay to Detect Schistosoma ViabilityThe blood fluke Schistosoma is a parasitic trematode that is responsible for more than 200,000 human deaths per year.  Most Schistosoma species are located in tropical and subtropical areas of the delveloping world. The state of the art to detect Schistosoma viability involves microscopy and knowledge of parasite morphology. The lack of appropriate methods for quantifying Schistosoma viability blocks the development of new anthelmintics.

In this application note we present a fluorescence intensitybased microtiter plate assay to reproducibly detect schistosomal viability using the POLARstar Omega from BMG LABTECH (Fig.1). The principle of the assay is based on differential membrane permeabilities of the dyes, fl uorescein diacetate (FDA) and propidium iodide (PI). Fluorescein diacetate is able to cross the membrane of living cells. Once inside the cell, esterases will cut the diacetate and fluorescein is released resulting in a measurable fluorescence signal that is directly related to the number of living cells. In contrast, propidium iodide cannot enter a viable cell. This dye will only stain the DNA of dead cells when the membrane has been compromised. The simultaneous detection of both propidium iodide and fluorescein diacetate measures allowed us to develop a fluorescence-based, microplate bioassay to improve detection of schistosomal viability. The assay is adaptive to 96-well and 384-well format, quantitative and provides objective readouts (relative fluorescence units) of parasite viability during in vitro culture. Using this flexible bioassay, we demonstrate its versatility in detecting schistosomal viability in response to a known inhibitor of thioredoxin glutathione reductase (auranofin).

To view the full article please click here


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events