The completely redesigned plan-apochromatic 20x/1.0 VIS-IR objective
lens from Carl Zeiss now makes it possible to acquire 3D images of nerve cells
down to depths of 5.6 mm in intact tissue using a confocal laser scanning or
multiphoton microscope, without having to section the brain. This permits the
three-dimensional visualization of the branches of individual nerve cells and
the imaging of their connections. In untreated tissue, the penetration depth
now achievable is five to ten times more than that of a multiphoton microscope
featuring traditional optics.
Using the microscope to visualize and track nerve cells deep into the
brain is an important step in the decoding of the brain circuitry. The new
objective lens from Carl Zeiss now brings this goal considerably closer to
realization. Carl Zeiss has designed the plan-apochromatic 20x/1.0 VIS-IR
objective lens for the clearing method known as Scale
developed by Dr. Atsushi Miyawaki at the RIKEN Brain Science
Institute in Japan.
A special water-based reagent solution transforms the sheath substance of the
nerve cells into a transparent matrix without impairing the signals from
fluorescent marker and tracer substances.
"I am very impressed by the new possibilities," says Dr. Atsushi
Miyawaki, whose results have been published in
Nature Neuroscience and provoked broad interest in the
neurosciences. In one of the most important research projects in the field of
brain research, the Connectome project, the objective lens will help scientists
obtain a better understanding of brain connectivity.
For more information visit
www.zeiss.com/microscopy
