Search News Archives
Social Media
Will BRIC be the Savior for the Life Sciences and Medical Devices Sector? Seal of Approval for Pepceuticals ZEISS Lightsheet Z.1 awarded with Best New Life Science Product of 2012 IDT and SGI-DNA Broaden Their Collaboration to Provide Synthetic DNA Products up to 2 Mbp Watson-Marlow OEM pumps help push the boundaries of automated cell culture Breakthrough in non-invasive gut health testing New Portable SPECTROSCOUT XRF Analyzer - the Lab that Goes Anywhere ZEISS SIGMA used to analyze Russian meteorite that fell in Chelyabinsk region
Waters Updates Alliance HPLC Designs Without Impacting Established Validated Methods Porvair Sciences Expands Product Range for Epigenetics Thermo Fisher Scientific Introduces Gas Chromatography Headspace Autosampler for Organic Volatiles New Amicon® Pro Purification System for Protein Purification from Merck Millipore Wyatt Calypso Succeeds in ABRF-MIRG Study Revolutionize your pipetting with the new Biohit Picus electronic pipette Easy, Reliable Weighing in Regulated Areas Phenom proX all-in-one desktop SEM Flash of inspiration in the shaft tunnel - IKA launches the new video for UTTD control The only Chromatography Autosampler you will ever need! JG Finneran Associates introduces the 96-Well Multi-Tier Microtiter Plate System (Patented) IC for the people – Metrohm celebrates 25 years of ion chromatography
For further information or to sign up to receive any of our E-Newsletters click here Reading and Imaging are Combined in BioTek's Revolutionary, New Cytation™3 ZEISS SIGMA used to analyze Russian meteorite that fell in Chelyabinsk region New SPOT Insight™ Gigabit Camera Brings Microscopy Presentations to Life ZEISS Lightsheet Z.1 awarded with Best New Life Science Product of 2012 Malvern’s Dr E Neil Lewis takes his place among chemical imaging pioneers Waters Biopharmaceutical, Bioanalysis and Screening Solutions Now Shipping with UNIFI 1.6 New Software for Research & Scientific Thermal Imagers Thermo Fisher Scientific Introduces Data System to Unite Chromatography and Mass Spectrometry Molecular Devices Introduces SoftMax Pro 6 GxP Microplate Data Acquisition and Analysis Software Short Guide to 3D Cell Culture New EFS Handbook Published by Lee Products The Perfect Ultrafiltration Device for Your Specific Need Electron Magnetic Resonance shrinks to fit the undergraduate curriculum Biotage Releases New Catalog and Technical Guide for Sample Preparation and Evaporation New Waters Quality Parts, Chromatography Columns and Supplies Catalog
|
Techne TC-PLUS thermal cycler is ideal for new fast PCR protocols
publication date: Jun 18, 2010
|
author/source: Phoenix Marcom Limited
Time savings are an advantage in many research labs and the Techne TC-Plus is the ideal tool for use when optimising for fast PCR protocols. This thermal cycler offers flexible programming and rapid block ramp rates of up to 5ºC per second. Comparing the same PCR program on the TC-Plus and the slower TC-512 gave a time saving of around 30 minutes simply by using faster ramping (1 hour compared with 1 hour 30 minutes). There were no appreciable differences in yield or sensitivity between the two thermal cyclers.* Another way to shorten run times is to modify the standard protocol by reducing the number of steps and/or hold times of individual steps. A temperature gradient optimisation experiment changing from three to two steps using the TC-Plus saved 22 minutes (52 minutes run time for the two-step protocol; 1 hour 14 minutes for three steps). There was a decrease in the amount of 400bp fragments, but no noticeable difference in yield of the 100bp PCR product.* Reagents specifically formulated for fast PCR contain specialised enzymes and stabilisers to facilitate rapid ramping and short hold times. Use of a fast PCR mix gave improved yield and sensitivity compared with the standard mix when running a fast protocol on the TC-PLUS. However, this was overall lower than when using a standard protocol with longer hold times for each step. The total run time using the fast protocol was around 40 minutes compared to the standard run time of 1 hour on the TC-PLUS or 1 hour 30 minutes on the TC-512.* According to Rob Skehens, Bibby Scientific Marketing Director, ‘The results in our new Application Note show that while the faster ramping of the TC-Plus alone gives significant time savings, modifying the program and changing to a fast PCR mix can shorten run times even further. If any resultant compromises in yield and sensitivity are unacceptable, some reaction optimisation may be needed.' * Bibby Scientific Application Note A01-003A For further information please visit www.techne.com |
|
With one of the fastest aluminium blocks on the market, the Techne TC-Plus thermal cycler has been shown to contribute to significant savings in PCR reaction times by using faster ramp rates, modified programs and modified reaction mixes.