Tecan's
Infinite
® M1000 plate reader has enabled researchers at the
Institute of Entomology (ENTU), Czech Republic, to automate hourly luminescence
measurement of transgenic
Drosophila for the study of insect circadian
clocks. Dr David Doležel, a researcher at ENTU, explained: "The expression of
circadian genes is studied using transgenic
Drosophila containing
luciferase reporter genes controlled by the promoter of the gene under study.
This requires a microplate reader that includes a luminescence module equipped
with filters to discriminate between red and green luciferases, allowing us to
follow multiple genes per well. Automatic plate transfer is also essential in
order for measurements to be performed at regular intervals throughout the day
and night."
David
continued: "The Infinite M1000's compact size means that it fits easily into
our laboratory incubator, so we can precisely control light and temperature and
investigate the effect of environmental variables. Its microplate format allows us to
load hundreds of flies into the plate reader at the same time, automating
large-scale screens of mutant
Drosophila strains and performing hundreds
of measurements over a period of up to two weeks. Overall, we have been very
impressed by the system's performance and flexibility."
To find
out more about Tecan's Infinite M1000 plate reader, visit
www.tecan.com/infinitem1000
