The Symbiosis^TM Pharma is Spark Holland's unique solution for integrated on-line SPE-LC-MS automation (XLCMS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes: LC-MS, XLC-MS, or AMD (Advanced Method Development). A sensitive, specific, accurate and precise assay was developed for the on-line extraction and determination of paclitaxel in human plasma by XLC-MS, using the Symbiosis^TM Pharma. This method was developed as part of an evaluation, to introduce this new technology into our bioanalytical laboratory. This application note demonstrates the ease of transfer of an off-line sample pretreatment to an on-line protocol, with a concomitant increase in productivity. Paclitaxel is a taxane diterpene amide that was first extracted from the Western yew, Taxus brevifolia. This compound has been shown to be effective in the treatment of a variety of human neoplastic diseases.

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