Syngene, a world-leading manufacturer of image analysis
solutions, today announced the results of a head-to-head study carried out at
the University of
Cambridge to demonstrate
that the G:BOX Chemi IR
6
can produce images of IR labelled Western blots up to 10 times faster than
laser based scanning.
The G:BOX
Chemi IR
6, which is Syngene's new multi-application CCD imaging
system for analysing IR and fluorescently stained proteins, was tested against
a laser based scanner. Both imagers were tested side-by-side in a comparison
study at the University of Cambridge for analysing proteins on Western blots
labelled with DyLight
® 680 and DyLight
® 800 dyes (Thermo
Fisher Scientific).
The results, detailed in Syngene's Application Note 62,
show that using the G:BOX Chemi IR
6
with Epi Red lighting and a 705nm filter, an image of DyLight 680 labelled
bands can be captured in just 20 seconds. With Epi LED IR740 lighting and a LY800
filter, an image of the DyLight 800 labelled proteins can be generated by the G:BOX Chemi IR
6 in two minutes. In
comparison, the laser based scanner can take more than 10 times longer to image
DyLight 680 and twice as long to image DyLight 800. The systems had comparable
sensitivity, with both their limits of detection being 0.11µg.
Lindsey Bunn, Syngene's Application Specialist explained:
"With laser-based scanners imaging times are determined by scanning resolution
and intensity setting, whereas using a G:BOX
Chemi IR
6, imaging times only depend on sensitivity. This is why we
believed our CCD system would be faster than and just as accurate as a laser
based system and are delighted that the results of the study have proved this."
Lindsey continued: "The added benefit with the G:BOX Chemi IR
6 is the system can analyse
all commercial fluorescent, chemiluminescent and visible dyes as well, making
it a more cost-effective purchase. Researchers who need to rapidly produce images
of multiplex labelled Westerns but whose labs don't have the budget for an
expensive laser scanner should consider testing a G:BOX
Chemi IR
6 system today."
For more information visit
www.syngene.com
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