Integrated DNA Technologies (IDT), the
world leader in oligonucleotide synthesis, provides a broad range of custom
qPCR assays, primers, and probes. PrimeTime
® qPCR Assays are
available in three different sizes (Mini, Standard, and XL) and provide the
forward and reverse primers, as well as a hydrolysis probe in a single tube.
Primers and probes are also available separately, including the new
Double-Quenched Probe
TM which increases accuracy and sensitivity through the
incorporation of an internal ZEN
TM quencher. As such, users are provided with an
option to meet any experimental requirements.
PrimeTime qPCR Assays allow users to select from multiple
dye-quencher combinations, as well as define primer:probe ratios to optimize
experimental set-up. Each oligo
undergoes 100% QC by mass spectrometry and is shipped in 2-4 days of order.
IDT offers PrimeTime Mini qPCR probes, which are ideal
for gene expression screens. Mini probes are offered with a 5' FAM and the
option of a 3' IBFQ quencher alone, or in combination with the internal ZEN
quencher. Users can also purchase Express probes, which are shipped in one
working day, making them ideal for time-sensitive laboratories.
IDT now also offers PrimeTime Pre-designed
Assays that are guaranteed to work for human, mouse, and rat. Users routinely
experience increased sensitivity and higher qPCR efficiency when using these
Assays. The design process uses the most current, clean, and complete target
sequence information available and stresses accurate T
m prediction
and protection against off-target amplification. The ordering system contains
Assays designed to target exon boundaries for every human, mouse, and rat
transcript in the NCBI database.
For more information on IDT's qPCR offering
and to use the free online Pre-designed
ordering tool or the RealTime PCR design tool for custom primer and probe
sequences, please visit
www.idtdna.com.
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