Market leaders in temperature controlled microscopy,
Linkam Scientific Instruments announce the use of their THMS600 temperature
stage at the Leiden University Medical Center to aid the cryo-study of
biological specimens.
Professor A.J. Koster (Leiden University Medical Center) is one of the founders
of NeCEN, the Netherlands Center of Electron Nanoscopy which was formed to
advance cryo-TEM in the Netherlands by joining national groups in the
realization of a national centre housing a number of high-end electron
microscopes (
www.necen.nl).
Professor Koster's own group focuses on applications in cell biology. His goal
is to localize molecular structures in cells using fluorescence microscopy and
then transfer the sample to a cryo-electron microscopy (Cryo-EM) set up to
image the corresponding macromolecular structures in 3D with nm-scale
resolution. Two areas are of particular interest: the study of viral infections
and viral replication where fluorescence may be used to pinpoint areas worthy
of enhanced investigation. The other is in the field of vascular biology to
study the process of regulated exocytosis of Weibel-Palade bodies (WPBs) that
is a pivotal mechanism via which vascular endothelial cells initiate repair in
response to injury and inflammation.
The goal of the studies was to develop a setup and workflow for cryo-CLEM
(correlative light and electron microscopy) that permits visualization of
structures in cryo-FM (fluorescence microscopy) at sub-cellular resolution, and
that retrieves those structures accurately in cryo-EM for the purpose of cryo
electron tomography. The group wanted a cryo-FM setup that was easy to
implement. To this end, Professor Koster and his colleagues opted for a
commercially-available heating and freezing stage (the Linkam THMS 600), which
was modified in order to accommodate EM support grids.
As Professor Koster says, "before we found the Linkam system in the
literature and the ability to correlate microscopies, combining modalities was
next to impossible. We have now been using this cryo-CLEM method for more than
three years. It has certainly enabled us to produce results quickly and hence
get to publication more rapidly too."
(European Journal of Cell Biology
88 (2009) 669-684).
More than 3,000 THMS600 stages are in use in laboratories worldwide. It is used
in many applications where high heating/freezing rates and 0.1°C accuracy and
stability are needed. Samples can be quickly characterized by heating to within
a few degrees of the required temperature at a rate of up to 150°C/min with no
overshoot, then slowed down to a few tenths of a degrees per minute to closely
examine sample changes.
Visit the
Linkam website (
www.linkam.co.uk)
today and learn about the broad range of applications in the field of
temperature controlled microscopy.
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