publication date: Mar 25, 2014
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author/source: Porvair Sciences Limited
Porvair Sciences' has produced an informative new guide entitled ‘Top 10 tips for positive ChIP results’.
The success of a Chromatin immunoprecipitation (ChIP) assay is highly dependent on the quality of chromatin prepared. Written by scientists with many years experience of ChIP, the new 8-page document provides an informative 10-point illustrated guide to help you improve your ChIP assay results. ChIP is a commonly used immunprecipitation technique for mapping the DNA-protein interactions in cells which are crucial for correct gene regulation. In a ChIP assay DNA-protein complexes (Chromatin) are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components.
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