TAP Biosystems, a leading supplier of innovative cell culture
systems and consumables for life science applications, today announced that a
peer reviewed paper authored by scientists at a leading US biopharmaceutical
company, has demonstrated that the advanced micro bioreactor system (ambr
TM)
system is equivalent in performance to a 2L bioreactor, showing that ambr is a
realistic scale-down model.
The ambr system, which closely replicates the characteristics of
bioreactors at micro scale, was tested in a fed-batch process with four
recombinant Chinese Hamster Ovary (CHO) cell lines expressing antibodies. The
clones were grown in parallel in ambr, shake flasks and 2L benchtop bioreactor
to determine which technology provided the optimum scale-down model for their
process development work. Their results showed that cultures grown in ambr
matched those grown in a 2L bioreactor, in terms of controlling the environment
and culture performance. However, cultures grown in shake flasks did not
compare to those grown in either ambr or the 2L bioreactor.
This publication also indicated that using ambr reduced manual
operations by automating many liquid handling and sampling functions. The paper
stated that this increased the throughput and operational efficiency and
ensured scientists at the company saved time using ambr compared to using a 2L
bioreactor as a scale-down model to predict antibody expressing CHO cell
performance in large scale bioreactors.
Dr Barney Zoro, ambr Product Manager at TAP Biosystems stated:
"This paper highlights literature reports and provides clear evidence
that, due to limitations in pH and DO control; shake flasks are a poor model
for process scale-up. By contrast, ambr is shown to be an equivalent model to
2L bioreactors, with automation and single use technologies making ambr both
easier and more time efficient for clone selection and scalable process
development."
Zoro concluded: "For CHO cell culture scientists focused on
rapidly developing robust antibody production processes, the data confirms that
using ambr as a scale-down model will provide them with a major competitive
advantage."
For more information visit
www.tapbiosystems.com
Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!
Request your free copies HERE
Popular this Month
Top 10 most popular articles this month
Today's Picks
Looking for a Supplier?
Search by company or by product
Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.
Lab Bulletin is published by newleaf marketing communications ltd.