Allowing Researchers to
Create Physiologically Relevant Collagen-Based Tissues
TAP
Biosystems, a leading supplier of innovative cell culture systems and
consumables for life science applications, today launched a new e-commerce site. The
new site will provide a fast and secure method for researchers in North America
and Europe to purchase RAFT 3D cell culture kits online, allowing them to
create realistic cell cultures incorporating cells of their choice.
For
the launch, the site will offer a special RAFT Starter Kit - ideal for
researchers using RAFT for the first time. The Starter Kit will contain
sufficient plates (clear or black walled), and sterile reagents, together with
a plate heater specially designed for use in a laminar flow hood, allowing the
simple production of up to four 96-well plates of consistent 3D cell
cultures. A standard, easy-to-follow protocol for producing the cultures
using RAFT is also provided.
The
site will also offer downloads of technical data on the RAFT system, as well as
applications information and current bibliography. These include a recent
poster presentation which demonstrates the range of analytical techniques which
have been applied successfully to RAFT cultures.
RAFT
is a new system which combines a novel patented process and a range of
easy-to-use consumables and reagents to create 3D cell cultures from collagen
in a convenient, easy-to-use format. Using the standard protocol
supplied, scientists simply mix the reagents from the Starter Kit with their
cells, pipette into 96-well plates and incubate on the plate heater for 15
minutes to form a cell-seeded collagen hydrogel. The custom RAFT plate
containing 96 absorbers is placed on top of the cell-containing hydrogels, and
the medium is absorbed gently, leaving cells "encapsulated" in physiological
strength collagen, perfect for use in cell-based assays.
Dr
Grant Cameron, TAP Biosystems' RAFT Development Director said: "We're excited
to offer scientists a quick, convenient method of accessing RAFT information
and purchasing this exceptional technology. As support for the new site, we
also have a team of dedicated RAFT application specialists available to discuss
any technical questions."
Cameron
continued: "The standard protocol we provide is an excellent starting point for
using the RAFT system. However, the system is so versatile that researchers can
modify conditions for their cells to accommodate their experimental design. For
example, they can change cell seeding densities and even add multiple different
cell types to produce their own co-cultures. In short, any scientist looking to
create their own reproducible 3D cell culture tissues should go to
www.raft3dcellculture.com."
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