Anasys
Instruments is pleased to report the publication and highlighting of a "hot
paper" from a group of leading French scientists in the leading scientific
journal, Angewandte Chemie. The paper address a molecular mapping challenge
using the exciting technique of AFM-IR, the combination of an atomic force
microscope (AFM) and IR spectroscopy.
The mapping of molecules inside cells is a contemporary challenge that requires
both high sensitivity and sub-micron resolution. IR-spectroscopy is valuable
for chemical imaging. In the case of vibrational excitation in the IR, no
photobleaching is induced in contrast to what is observed with organic
fluorophores in the visible or UV range. The diffraction limit restricts
optical resolution in the IR to over 5 μm. The paper describes how this
challenge was overcome using thermal rather than optical detection.
The AFM-IR technique was developed by Dr. Alexandre Dazzi at the University of
Paris-Sud. It uses an AFM-tip in contact mode with an object as the IR
absorbance detector. This breakthrough technique made it possible for this
consortium of chemists, physicists and cell biologists to localize a
rhenium-carbonyl complex inside cells after a 1h-incubation at 10 μL. They have
also localized the nucleus using its own IR-signature without any trackers and
shown that the molecule is localized inside the nucleus.
Dr. Dazzi's research has been at the core of the Anasys nanoIR system.
Potential nanoIR application areas include polymer blends, multilayer films and
laminates, organic defect analysis, tissue morphology and histology, subcellular
spectroscopy, and organic photovoltaics. For further details, please see the
application notes at the
Anasys website
Reference:
Subcellular Imaging in the mid-IR of a Metal-Carbonyl Moiety
using Photothermal Induced Resonance, Clotilde Policar, Jenny-Birgitta
Waern, Marie-Aude Plamont, Sylvain Clède, Céline Mayet, Rui Prazeres,
Jaan-Michel Ortega, Anne Vessières, Alexandre Dazzi,
Angew. Chemie, Int.
Ed., 2011, 860.
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