Sartorius Stedim Biotech (SSB) today announced a new paper entitled, ‘Shortening Timelines for Upstream Bioprocessing of Protein-based Therapeutics’ has been published in the journal, BioPharma Asia.
The paper details how scientists at leading Asian pharmaceutical company, WuXi AppTec are using the ambr® 15 automated micro bioreactor system to mimic bench-top and production scale bioreactors, producing comparable results with cell growth, cell metabolism and protein production, including similar protein titre to BIOSTAT® STR single-use bioreactors.In the article, one case study describes how...
A new customer technical note compares the performance of the INTEGRA VOYAGER adjustable tip spacing multichannel pipette with a manual single channel pipette for performing real-time PCR experiments.
In undertaking these experiments, independent researchers found that the unique electronically adjustable tip facility on the VOYAGER not only generated time savings of up to 80% from minimising the number of required manual handling steps but also improved pipetting reproducibility and reduced pipetting errors.
Synbiosis,a long-established, expert manufacturer of automated microbiological systems, is delighted to announce a new paper entitled, ‘Evaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories’ has been published in the peer-reviewed journal, Methods X.
The paper details how scientists at the Australian Water Quality Centre (AWQC) have for the first time independently validated that results from the ProtoCOL 3 automated colony counter are as accurate as manual colony counting. The article describes how microbiologists at AWCQ, a leading NATA (National Association of Testing Authorities) accredited water testing laboratory in Adelaide, Australia cultured microorganisms isolated from...
INTEGRA has published a new white paper that discusses how observing good pipetting practice helps to achieve better results in serial dilution assays.
Preparation of reproducible serial dilution assays, especially in 384-well plates, is a challenging task because they require many pipetting actions and are highly dependent on an operator's technique and experience. However, observing good pipetting practice helps immensely to improve reproducibility and accuracy. In this white paper the author focuses on a selection of good pipetting techniques, which if maintained offer the best chance of...
A new technical poster produced by Genevac, in conjunction with the Novartis Institute for BioMedical Research (Horsham, UK), examines the application of eXalt™ evaporative crystallisation technology to drug discovery projects.
eXalt™ is a proprietary Genevac technology designed to control evaporation using restricting baffles. Using this technology multiple solvents can be screened in parallel to identify optimum crystallisation conditions for amorphous materials. In the pharmaceutical industry, physical form can pose a significant challenge for small molecule drug discovery projects transitioning between the optimisation and characterisation phases. A crystalline form is desirable...
Sphere Medical publishes white paper discussing areas where Proxima mitigates sources of pre-analytical errors
Sphere Medical, innovator in critical care monitoring and diagnostics equipment, has published a white paper focusing on the mitigation of common pre-analytical errors associated with arterial blood gas analysis [1]. The informative paper is available for download from Sphere’s online clinical resource centre and explores how and why pre-analytical errors occur, as well as the impact of an error on analyte levels in the blood sample. It also considers the...
Chromatrap®, a business unit of Porvair Sciences, has published a new application note that describes how Chromatrap® ChIP-seq assays now enable unbiased, genome-wide understanding of protein-DNA regulatory networks.
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how the Chromatrap...
Micronic has published a technical note that describes a study in which the protein binding properties of commercially available sample storage tubes was tested.
Driven by the rapid growth in proteomics and protein research applications the need for storage tubes where interaction between the sample and the tube surface is minimised has grown exponentially. The need for storage tubes that exhibit ultra-low binding properties is critical to these research fields due to the low concentrations of proteins typically found in samples. In the study – protein binding measurement tests were performed on Micronic...
The white paper “Adopting Next Generation Labeling Technology Enhances Enterprise Agility and Reduces Costs” published by NiceLabel identifies the hidden costs, inefficiencies and risks resulting from legacy labeling operations and explains why a modern label management platform is well suited to current enterprise and extended supply chain needs.
In today’s networked economy, organizations are challenged with volatile market requirements, massive connectivity driven by the "Internet of Things", and increased product, process, and supply chain complexity. Enterprises are continually looking deeper into their operations and farther across their supply chains for opportunities to become more efficient. Labeling is frequently considered a background process that does not get...
Chromatrap®, a business unit of Porvair Sciences, has published a new application note that describes how Chromatrap® ChIP-seq assays now enable unbiased, genome-wide understanding of protein-DNA regulatory networks.
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how...
Positive displacement pipettes have been used for many years for PCR applications. Gilson, experts in liquid handling have published three new papers which describe the use of positive displacement technology to help solve the issues of pipetting problem liquids.
Gilson’s MICROMAN E positive displacement pipette with its Capillary Pistons helps scientists counter common pipetting problems that may affect accuracy and precision when working with problem liquids. Leaks are known to occur when pipetting volatile solutions such as acetone, hexane and ethanol with an air-displacement pipette.
‘Extending the boundaries of QbD’, a new whitepaper from Malvern Instruments, explores the application of Quality by Design (QbD) principles to the development of analytical instrumentation and the benefits this brings for users.
Taking the example of Malvern’s development of the Mastersizer 3000 particle size analyzer, the authors illustrate how instrument companies are aligning with the QbD philosophy. This offers pharmaceutical customers the reassurance of closely similar working practices and products that meet exacting customer standards. The central idea of QbD is that quality should be built into a product from the outset. It is an approach based on...
The incredibly powerful potential of photochemistry is revealed in four application notes recently posted by Suffolk-based flow chemistry engineering firm Vapourtec.
The four application notes cover 'Synthesis of Artemisinin using E-Seriesand UV-150 reactor' (Application Note 39), 'α-Photodecarboxylation of Phthaloyl Glycine' (38), 'Photochemical Transformation of Methyl Coumalate' (37) and '[2+2] Photocycloaddtion of Maleimide and 1-Hexyne' (36) and are available to download in full from the Vapourtec website. Vapourtec MD and founder Duncan Guthrie explained: "Taken together these...
Porvair Sciences, in conjunction with researchers at Swansea University (UK) have written a new technical article entitled 'Chromatrap® 96: a new solid-state platform for high-throughput Chromatin Immunoprecipitation (ChIP)'
Epigenetics is a fast-growing research area with wide applications, including disease mechanism profiling and personalized medicine strategies. ChIP is crucial to epigenetic research. Published in the latest issue of Nature Methods (11, 2014) the article provides an introduction to Chromatrap® 96 - a high-throughput ChIP analysis platform and demonstrates how it is able to profile up to 96 transcription factors and epigenetic modifications...
Exeter Analytical has published an application note demonstrating the utility of its Model 440 CHN microanalysis system for precise determination of percentage Carbon, Hydrogen and Nitrogen content of often demanding liquid samples.
The analysis of liquid samples, including volatile liquids, can be done quite easily on the Exeter Analytical Model 440 system without sacrificing accuracy or precision and without the need for expensive liquid autosamplers. The new application note 233 describes how a special liquid sample preparation technique together with controlled introduction of the sealed capsules into the Model 440 eliminates the problems associated with...
A new white paper from Copley Scientific provides expert guidance on how to use breathing simulators effectively when testing orally inhaled products (OIPs).
Breathing simulators are machines that mimic the inhalation and/or exhalation profile of a patient and are used increasingly in OIP testing. Copley Scientific’s white paper clearly explains where breathing simulators are specified by pharmacopoeial methods and also where their optional use is beneficial. This may be in improving understanding of product performance, for example, or to support a Quality by Design (QbD) approach...
Read MoreCLSI Releases New Standard on Laboratory Internal Audit ProgramsMar 4, 2014
The Clinical and Laboratory Standards Institute (CLSI) has released a new quality management systems standard on laboratory internal audit programs.
A laboratory audit program is critical to ensuring laboratories meet applicable requirements for quality system essentials assessments. QMS15-A—Laboratory Internal Audit Program; Approved Guideline provides guidance for how a laboratory can establish an internal audit program to enhance the quality of its services through continual improvement. Whereas an audit program defines the “who,” “what,” “when,” “where,” and “how” of meeting requirements for internal auditing, the audit process describes the details of how to conduct individual laboratory internal audits...
Frederic Girard, CEO of Spinnovation Biologics, has authored a new article detailing the benefits of novel Spedia-NMR™ and NMR technology for Quality by Design (QbD) process development within biologics production.
Entitled ‘Fingerprinting Culture Media’, the article was published in January 2014’s edition of European Biopharmaceutical Review and is now available for download online. The QbD initiative is rapidly being adopted throughout the conventional and bio-pharmaceutical industry to ensure product quality and consistency within manufacture. Exploring the background of the growing QbD movement within biotechnology....
Freeman Technology, a global provider of powder testing instruments, and GEA Pharma Systems, a company that specialises in the design and development of processing solutions for the pharmaceutical industry, are collaborating to advance the application of continuous wet granulation and drying technology in the pharmaceutical industry.
The work involves Freeman Technology’s FT4 Powder Rheometer® and GEA Pharma Systems’ ConsiGma™ 1 continuous granulation unit. Data from the FT4 are being used to quantify the influence of the operating conditions of the ConsiGma1 on the bulk characteristics of the granules being manufactured. These data are then correlated to attributes of the tablets, providing the link between granule and tablet properties.....
Read MoreNew whitepaper highlights impact of NGS target enrichment assay choiceDec 17, 2013
OGT provides insight on NGS enrichment optimisation
Oxford Gene Technology (OGT), provider of innovative genetics research and biomarker solutions to advance molecular medicine, has produced a free whitepaper titled ‘The importance of enrichment assay choice and optimisation for confident variant detection.’ The paper investigates the main strategies employed to optimise target enrichment for next generation sequencing (NGS) assays, assisting researchers to make informed decisions when designing studies...
A new application note from Malvern Instruments explores the role of particle size and the importance of particle size measurement in optimizing the formulation of drilling muds used in oil extraction processes.
The particle size of drilling mud components exerts a significant influence on the performance of the final drilling mud product. Rapid, reliable measurement and control of particle size is especially important when tailoring drilling mud formulations for individual geological situations and drilling operations. This new publication focuses on the use of laser diffraction particle sizing for this application, exploring the steps involved and providing examples of typical data generated.....
Read MoreProtocol for Preparing Top Quality Lyophilised SamplesDec 11, 2013
Genevac has developed a protocol using its SuperCool 75 condenser
Genevac has developed a protocol using its SuperCool 75 condenser that, in conjunction with the Genevac HT-12 High Throughput Evaporator, has been proven to set a new benchmark for enhanced evaporation and lyophilisation results. The SuperCool 75 chills to -75C and operates at higher power than any other commercially available condenser, opening the door to greatly improved results with the Genevac LyoSpeed fast lyophilisation process for preparing powdered samples from HPLC fractions...
The paper details how scientists at leading Asian pharmaceutical company, WuXi AppTec are using the ambr® 15 automated micro bioreactor system to mimic bench-top and production scale bioreactors, producing comparable results with cell growth, cell metabolism and protein production, including similar protein titre to BIOSTAT® STR single-use bioreactors.In the article, one case study describes how...
In undertaking these experiments, independent researchers found that the unique electronically adjustable tip facility on the VOYAGER not only generated time savings of up to 80% from minimising the number of required manual handling steps but also improved pipetting reproducibility and reduced pipetting errors.
The paper details how scientists at the Australian Water Quality Centre (AWQC) have for the first time independently validated that results from the ProtoCOL 3 automated colony counter are as accurate as manual colony counting. The article describes how microbiologists at AWCQ, a leading NATA (National Association of Testing Authorities) accredited water testing laboratory in Adelaide, Australia cultured microorganisms isolated from...
Preparation of reproducible serial dilution assays, especially in 384-well plates, is a challenging task because they require many pipetting actions and are highly dependent on an operator's technique and experience. However, observing good pipetting practice helps immensely to improve reproducibility and accuracy. In this white paper the author focuses on a selection of good pipetting techniques, which if maintained offer the best chance of...
eXalt™ is a proprietary Genevac technology designed to control evaporation using restricting baffles. Using this technology multiple solvents can be screened in parallel to identify optimum crystallisation conditions for amorphous materials. In the pharmaceutical industry, physical form can pose a significant challenge for small molecule drug discovery projects transitioning between the optimisation and characterisation phases. A crystalline form is desirable...
Sphere Medical, innovator in critical care monitoring and diagnostics equipment, has published a white paper focusing on the mitigation of common pre-analytical errors associated with arterial blood gas analysis [1]. The informative paper is available for download from Sphere’s online clinical resource centre and explores how and why pre-analytical errors occur, as well as the impact of an error on analyte levels in the blood sample. It also considers the...
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how the Chromatrap...
Driven by the rapid growth in proteomics and protein research applications the need for storage tubes where interaction between the sample and the tube surface is minimised has grown exponentially. The need for storage tubes that exhibit ultra-low binding properties is critical to these research fields due to the low concentrations of proteins typically found in samples. In the study – protein binding measurement tests were performed on Micronic...
In today’s networked economy, organizations are challenged with volatile market requirements, massive connectivity driven by the "Internet of Things", and increased product, process, and supply chain complexity. Enterprises are continually looking deeper into their operations and farther across their supply chains for opportunities to become more efficient. Labeling is frequently considered a background process that does not get...
Genome-wide mapping of protein-DNA interactions is essential for a complete understanding of gene regulation. A detailed map of epigenetic marks and transcription factor binding is necessary for deducing the regulatory networks that underpin gene expression in a variety of biological systems. The most widely used tool for examining these interactions is ChIP followed by massively parallel sequencing (ChIP-seq). The new application note reports on how...
Gilson’s MICROMAN E positive displacement pipette with its Capillary Pistons helps scientists counter common pipetting problems that may affect accuracy and precision when working with problem liquids. Leaks are known to occur when pipetting volatile solutions such as acetone, hexane and ethanol with an air-displacement pipette.
Taking the example of Malvern’s development of the Mastersizer 3000 particle size analyzer, the authors illustrate how instrument companies are aligning with the QbD philosophy. This offers pharmaceutical customers the reassurance of closely similar working practices and products that meet exacting customer standards. The central idea of QbD is that quality should be built into a product from the outset. It is an approach based on...
The four application notes cover 'Synthesis of Artemisinin using E-Seriesand UV-150 reactor' (Application Note 39), 'α-Photodecarboxylation of Phthaloyl Glycine' (38), 'Photochemical Transformation of Methyl Coumalate' (37) and '[2+2] Photocycloaddtion of Maleimide and 1-Hexyne' (36) and are available to download in full from the Vapourtec website. Vapourtec MD and founder Duncan Guthrie explained: "Taken together these...
Epigenetics is a fast-growing research area with wide applications, including disease mechanism profiling and personalized medicine strategies. ChIP is crucial to epigenetic research. Published in the latest issue of Nature Methods (11, 2014) the article provides an introduction to Chromatrap® 96 - a high-throughput ChIP analysis platform and demonstrates how it is able to profile up to 96 transcription factors and epigenetic modifications...
The analysis of liquid samples, including volatile liquids, can be done quite easily on the Exeter Analytical Model 440 system without sacrificing accuracy or precision and without the need for expensive liquid autosamplers. The new application note 233 describes how a special liquid sample preparation technique together with controlled introduction of the sealed capsules into the Model 440 eliminates the problems associated with...
Breathing simulators are machines that mimic the inhalation and/or exhalation profile of a patient and are used increasingly in OIP testing. Copley Scientific’s white paper clearly explains where breathing simulators are specified by pharmacopoeial methods and also where their optional use is beneficial. This may be in improving understanding of product performance, for example, or to support a Quality by Design (QbD) approach...
Entitled ‘Fingerprinting Culture Media’, the article was published in January 2014’s edition of European Biopharmaceutical Review and is now available for download online. The QbD initiative is rapidly being adopted throughout the conventional and bio-pharmaceutical industry to ensure product quality and consistency within manufacture. Exploring the background of the growing QbD movement within biotechnology....
The work involves Freeman Technology’s FT4 Powder Rheometer® and GEA Pharma Systems’ ConsiGma™ 1 continuous granulation unit. Data from the FT4 are being used to quantify the influence of the operating conditions of the ConsiGma1 on the bulk characteristics of the granules being manufactured. These data are then correlated to attributes of the tablets, providing the link between granule and tablet properties.....
Oxford Gene Technology (OGT), provider of innovative genetics research and biomarker solutions to advance molecular medicine, has produced a free whitepaper titled ‘The importance of enrichment assay choice and optimisation for confident variant detection.’ The paper investigates the main strategies employed to optimise target enrichment for next generation sequencing (NGS) assays, assisting researchers to make informed decisions when designing studies...
Genevac has developed a protocol using its SuperCool 75 condenser that, in conjunction with the Genevac HT-12 High Throughput Evaporator, has been proven to set a new benchmark for enhanced evaporation and lyophilisation results. The SuperCool 75 chills to -75C and operates at higher power than any other commercially available condenser, opening the door to greatly improved results with the Genevac LyoSpeed fast lyophilisation process for preparing powdered samples from HPLC fractions...