Avoiding extra column volume and unwanted peak
dispersion
High resolution UHPLC
systems are extremely sensitive to the introduction of extra column volume
(dead volume), which will adversely affect your chromatography and result in
unwanted peak dispersion. Therefore, when installing any UHPLC column, it is
vital to ensure that the high pressure inlet tubing is fitted into the column
port to the correct depth to avoid the introduction of any extra column volume.
The incorrect positioning of the inlet tubing, ferrule and nut can easily
result in up to a 23% loss in efficiency and an 11% deterioration in asymmetry
for your chromatography.
Advanced
Chromatography Technologies Limited have produced a new leaflet on ‘Making Good
UHPLC Column Connections'. The flyer explains how to ensure that a good UHPLC column
connection is made every time and details the importance of ‘making a fresh
connection' when installing any new UHPLC column. Features and benefits of the new
ACE range of reusable UHPLC column connectors are also discussed.
ACE UHPLC reusable
column connectors are designed to connect your UHPLC column to your UHPLC
system and make other similar connections. They are suitable for use with
1/16" OD tubing and virtually all manufacturers' UHPLC systems and all
brands of UHPLC columns. These compact, one-piece design fittings are simple
and easy to use. Installed correctly these fittings are non-permanent swaging,
protecting your valuable time and equipment. They are reusable and will provide
approximately 10 make and break cycles.
