Preheating of mobile phases - "nice-to-have" or "need-to-have"?
publication date: Dec 30, 2009
Controlling the temperature of a column is a prerequisite for reproducible results in HPLC.
Column ovens are part of most HPLC systems and many of them offer space even for semi preparative columns. But with increasing inner diameter the heat transfer from the air surrounding the column to the whole cross section of the column (filled with the cold mobile phase) becomes more and more difficult. This may result in bad peak shapes, as the radial temperature gradientcauses different separation conditions for every part of the sample depending on its distance to the outer diameter.
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