Members Login
Channels
Special Offers & Promotions
How to choose the correct pore size for oligonucleotide analysis by SEC
Pore size selection for separation of oligonucleotides by YMC-Pack Diol columns
SEC is a suitable method for the analysis of long chain oligonucleotides with length up to 200mer. The choice of the optimal pore size is crucial for the success of the method. In this Application Note, analyses of oligonucleotides of various lengths are described and a selection guide for columns for different DNA is discussed - a practical addition to your local application database.
Oligonucleotides cover a broad field of applications ranging from molecular biology to therapy and diagnosis. Some oligonucleotides, such as antisense oligonucleotides, act as single-strands, but also double stranded oligonucleotides exist, such as the case of small interfering RNA (siRNA) du-plexes. So far, the oligonucleotide length usually ranges from 12–25mer; however, the demand for longer oligonucleotides with lengths up to 200mer is increasing. These extremely long oligonucleotides are used in a variety of molecular biology applications including site directed mutagenesis.
Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!
Popular this Month
Top 10 most popular articles this month
Today's Picks
Looking for a Supplier?
Search by company or by product
Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.
Lab Bulletin is published by newleaf marketing communications ltd.
Media Partners