Agilent
Automation Solutions has released a new application note that demonstrates how
its Bravo liquid handling system provides an efficient platform for automation
of a SISCAPA Magnetic Bead Workflow for protein biomarker quantification by
Mass Spectrometry.
Mass Spectrometry (MS) has become the benchmark technology for analysis of
peptides based upon its ability to deliver precise quantification, near
absolute structural specificity and multiplexing of measurements in a single
analytical run. These advantages can be exploited in the analysis of peptides
which occur in a single sequence to reveal the concentration of the parent
proteins in digests of complex samples such as patient plasma or serum.
The authors of this new application note describe an automated protocol
implementing SISCAPA immunoaffinity enrichment of biomarker peptides prior to
quantification by MS. In the described protocol, magnetic beads coated with anti-peptide
antibodies were used to bind specific target peptides from tryptic digests of
plasma and serum samples, after which the beads were washed to remove unbound
peptides, and the bound, purified peptides were eluted in small volumes for
injection and analysis in an LC/MS/MS system. Stable isotope labeled
versions of the same peptides was used as internal standards to enable accurate
quantification. The Agilent Bravo automated protocol for this application
enabled SISCAPA processing of 96 samples in less than 30 minutes, less than
half the time required by traditional manual methods.
For a copy of this application note
click here or contact your local Agilent sales office.
From stand-alone units to fully integrated systems, Agilent Automation
Solutions teams key sample preparation and creative walk-away automation
approaches with personalized customer service to provide complete solutions for
your laboratory. Combining innovative engineering with high standards of
quality, Agilent designs and manufactures high-performance equipment for
processes that are revolutionizing pharmaceutical, biotech, and genomic
research.
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