Thermo Fisher Scientific is pleased to announce
a new method that uses
high-performance liquid chromatography (HPLC)
combined with
charged aerosol detection (CAD) to enable
improved quantitation of triterpene content in gotu kola. Low-wavelength UV detection
commonly used for triterpene analysis often results in strongly sloping
baselines, and interferences from minor components can adversely affect
quantitation. Customer Application Note (CAN) 111:
Determination of Triterpenes in Centella asiatica (Gotu Kola) by HPLC-CAD demonstrates
that charged aerosol detection minimizes these interferences and improves the
baseline, thereby enabling improved quantitation of triterpenes in gotu kola.
Gota kola is a small herbaceous annual plant
used as a medicinal herb in Ayurvedic medicine and used in traditional Chinese
medicine to treat a wide variety of conditions including improvement of memory
and blood flow. Gota kola is also used as a topical application for skin
conditions such as ulcers, wounds, and eczema. The chemical compounds of
interest in gotu kola are usually considered to be the ursane- and
oleanane-type triterpenes and the triterpene glycosides including asiaticoside,
madecassoside (asiaticoside A), asiaticoside B, madecassic acid, asiatic acid,
and terminolic acid.
This customer application note and many others
can be found at
www.thermoscientific.com/dionex
under the
Documents tab.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. is the world
leader in serving science. Our mission is to enable our customers to make the
world healthier, cleaner and safer. With revenues of $12 billion, we have
approximately 39,000 employees and serve customers within pharmaceutical and
biotech companies, hospitals and clinical diagnostic labs, universities,
research institutions and government agencies, as well as in environmental and
process control industries. We create value for our key stakeholders through
three premier brands, Thermo Scientific, Fisher Scientific and Unity
TM Lab
Services, which offer a unique combination of innovative technologies,
convenient purchasing options and a single solution for laboratory operations
management. Our products and services help our customers solve complex
analytical challenges, improve patient diagnostics and increase laboratory
productivity.
All trademarks are
the property of Thermo Fisher Scientific Inc. and its subsidiaries.
