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DETERMINATION OF PACLITAXEL IN HUMAN PLASMA BY XLC-MS USING SYMBIOSIS PHARMA

The SymbiosisTM Pharma is Spark Holland's unique solution for integrated on-line SPE-LC-MS automation (XLCMS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes: LC-MS, XLC-MS, or AMD (Advanced Method Development). A sensitive, specific, accurate and precise assay was developed for the on-line extraction and determination of paclitaxel in human plasma by XLC-MS, using the SymbiosisTM Pharma. This method was developed as part of an evaluation, to introduce this new technology into our bioanalytical laboratory. This application note demonstrates the ease of transfer of an off-line sample pretreatment to an on-line protocol, with a concomitant increase in productivity. Paclitaxel is a taxane diterpene amide that was first extracted from the Western yew, Taxus brevifolia. This compound has been shown to be effective in the treatment of a variety of human neoplastic diseases.

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