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Separation of oligosaccharides, including protein-linked glycans and glycopeptides

publication date: Apr 28, 2015
author/source: Hichrom Limited

New HALO Glycan HILIC Columns

HaloThe new HALO Glycan HPLC stationary phase is a high performance material specifically targeted for separations of highly polar oligosaccharides, and particularly protein-linked glycans and glycopeptides, by hydrophilic interactive liquid chromatography (HILIC).

The phase has a highly polar ligand containing 5 hydroxyl groups, which is attached to a 2.7micron Fused-Core silica particle via novel proprietary linkage chemistry. The Fused-Core particle used for HALO Glycan has a surface area of ~135 m2/g and an average pore size of 90Å.

HALO Glycan columns are part of the innovative HALO BioClass family of Fused-Core columns from Advanced Materials Technology. This range also includes HALO Protein columns (C4 & ES-C18 phases) and HALO Peptide columns (ES-C18 & ES-CN phases) which aims to provide chromatographers with a wide range of application specific solutions for the separation of bio-derived samples.

For further information on HALO Glycan and HALO BioClass columns, please contact authorised distributor Hichrom Limited on Tel: 0118 930 3660 or

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