Porvair Filtration Group has
announced that the Childrens Research Institute at the Medical College of
Wisconsin (
www.chw.org) has used the company's
Chromatrap
TM technology to develop more efficient and more precise Chromatin
Immunoprecipitation (ChIP) assays.
The Children's Research Institute is renowned for its state-of-the-art
pediatric studies. The CRI's translational research programs are targeted
to find life-saving discoveries and cures in the diseases that affect children
and interventions that enhance quality of life for children and families living
with chronic health conditions.
Over the last few months - Dr Ronald N Hines, Professor of Pediatrics,
Pharmacology & Toxicology at the CRI has compared the efficacy of
Chromatrap
TM with traditional bead based ChIP technologies in his research into
known regulatory elements controlling ontogeny of important human drug
metabolizing enzymes, such as CYP3A4. He commented "I believe the
bead-based system gave us a relatively small signal to noise ratio such that
even minor deviations caused by variation in the efficiency of the bead system
(for whatever reason) resulted in considerable variation in the
assay". He added "By comparison I think the most substantial
advantage of the Chromatrap system is more efficient and less variable trapping
of the immunoprecipitated chromatin such that the signal to noise ratio is
larger and influenced less by minor variations in trapping efficiency".
The Chromatrap
TM spin column approach offers significant advantages compared to
methods based on agarose or magnetic beads, which involve many steps of
separation, pipetting and re-suspension. The BioVyon
TM protein A discs used in
Chromatrap
TM spin columns have a proprietary internal structure, composed of
pores that have inner surfaces specifically designed to maximize chromatin
capture efficiency. Porvair's proprietary disc base material is chemically
inert, reducing incidences of non-specific binding. All chromatin capture
reactions take place inside the rigid porous disc; therefore there is no need
for the many repetitive stages of suspension, collection and resuspension by
pipetting. Additionally no pre-blocking step is required. The Chromatrap spin
column protocol allows washing steps that are fast, efficient and less prone to
error and the 'flow through' characteristics of the column ensure excellent
sample mixing and washing during the assay.
For further information please visit
www.chromatrap.com
or contact Porvair Filtration Group on +44-1489-864330 /
kevin.quinlan@chromatrap.com.
Porvair Filtration Group is an international leader in the development and
supply of materials and products for filtration and separation applications.
Their expertise in a wide range of media and the dedication of their design, manufacturing,
sales, test and research teams ensure they are at the forefront of filtration
technology, delivering world class performance to the Life Science, Aerospace,
Defence, Nuclear, Energy, Chemical Process, Industrial Process, Water Treatment
and Printing markets. With offices and manufacturing sites located in the
UK, Europe and the USA combined with a world-wide network of market
representatives and distributors, they pride themselves on their continuous
innovation and research to meet global demands.
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