Follow us...

 

Search News Archives

Monthly Newsletter

 

Lab Bulletin May Newsletter

view the latest issue

Subscribe

News Channels

 

New Laboratory Products

 

Lab News

 

Microscopy & Image Analysis

 

Separation Science

 

Research & Case Studies

 

Literature

 

Videos

 

Events | Webinars

 

 

 

Conferences | Events

DELMIC Reports on a New Review Paper Published in Nature Methods on Correlated Light & Electron Microscopy from their User Groups at the Universities of Delft and Groningen

publication date: Jul 24, 2015
 | 
author/source: Delmic BV

DELMIC develops and manufactures products which are focused on high performance, user friendly, integrated microscopy solutions.

high performance fluorescence SECOM platformUsers of Delmic's SECOM solution for Correlated Light & Electron Microscopy (CLEM) have recently published a review in Nature Methods that illustrates the power of CLEM in the world of biology. 

The boom in the use of super-resolution microscopies in recent years reached a peak with the award of the 2014 Nobel Prize in Chemistry to Betzig, Hell and Moerner for their contributions in the development of super-resolved fluorescence microscopy. Bridging technologies to advance the possibilities of a better understanding of biological processes has driven researchers to new capabilities. Light microscopy has been used since the mid seventeenth century to observe living microorganisms and cells. Later, the use of fluorescent dyes enabled the localisation of molecules within living cells. In parallel, electron microscopy (EM) was used to study cellular ultrastructure. Now, these microscopies have been integrated in a new technique called CLEM – Correlated Light and Electron Microscopy. 

Pascal de Boer and Ben Giepmans from the Department of Cell Biology at the University of Groningen together with Jacob Hoogenboom from the Faculty of Applied Sciences at Delft University of Technology have just published a review of CLEM with the encompassing sub-title of "ultrastructure lights up!" The review is published in Nature Methods1 and is now available online. 

The paper highlights the rapid development and growth of CLEM. Previously, fluorescence and EM experiments on the same sample were performed sequentially. Now, it is possible to perform these in integrated systems such as the SECOM platform.2 The authors illustrate this with some work from Christopher Peddie and Lucy Collinson from Cancer Research UK. This shows integrated microscopy (using the SECOM system) of resin-embedded HeLa cells expressing GFP-C1, a diacylglycerol sensor. The overlaid data shows excellent correlation between the fluorescence and EM images clearly illustrating the power of using such techniques in the future. As the authors state, "CLEM adds resolution and cellular context to LM observations and adds dynamics and target identification to EM observations. Experiments based on CLEM are beginning to provide insight in several biological contexts." 


more about delmic


 



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners