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EKF Molecular Diagnostics and GILUPI Announce Circulating Tumour Cell DNA Enrichment and Mutation Detection from Single Figures of Cells

publication date: Jun 20, 2014
 | 
author/source: EKF Diagnostics

EKF Molecular Diagnostics, and GILUPI, an innovator in medical devices for in vivo isolation of rare cells directly from a patient’s blood stream, announce results from collaborative work using the GILUPI CellCollectorTM and EKF Molecular’s PointManTM DNA Enrichment technology.

EKF LogoThe first results of the collaboration have successfully demonstrated the detection of gene mutations from as few as three or less cells isolated in a model in vitro system and from the blood of lung cancer patients.  PointMan DNA Enrichment was used to detect and analyse cells with known mutation status that had been collected on GILUPI CellCollectors under laboratory conditions.  The known mutations were those typically seen in lung (EGFR) and colorectal (KRAS) cancer.  Positive results using PointMan assays for KRAS (codon 12/13) and EGFR (T790M and L858R) from cell lines with known mutations and patients were confirmed by Sanger sequencing and showed conformance with known mutation status.

Andy Webb, CEO of EKF Molecular Diagnostics commented “These results are a clear indication of the utility of PointMan DNA enrichment on low cell numbers, in this case those isolated using the GILUPI CellCollector both in vitro and in vivo.  We look forward to continuing this important work with GILUPI to further validate our findings. This achievement is in line with the Company’s vision to change current DNA extraction and detection practices and address the fast growing companion diagnostics market.  Indeed, these new results compliment previous work on circulating free DNA using PointMan detection, which was performed by the Institute of Life Sciences, Swansea, UK, using samples archived in the Wales Cancer Bank.  The GILUPI and Swansea collaborations focus on the unmet requirements for patient monitoring from peripheral samples, negating the requirement for a surgical procedure to obtain a tissue biopsy for early cancer screening, diagnosis and during disease progression.”

Klaus Luecke, CEO and co-founder of GILUPI said “This is a major step forward for both companies and also for the future testing and monitoring of cancer patients.  The isolation and subsequent characterisation of low numbers of circulating tumour cells from the blood of cancer patients will become increasingly important as a liquid biopsy as a method to monitor disease progression and response to therapy.  We hope that less-invasive and more frequent testing will become routine using our combined technologies with significant patient benefits.  GILUPI is looking forward to further cooperation with EKF Molecular Diagnostics to continue to build the evidence base for the combined technologies.”

The preliminary results will be available for discussion at the EKF Booth at ASCO (May 30th – June 3rd 2014, Booth 4109, McCormick Place, Chicago, IL).


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