Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

Florida State University Demonstrates How Using Appropriate Buffer Conditions Improves Sample Preparation for Biophysical Studies - Such As Structural Analysis

New Customer Application Note Available from Wyatt Technology

DynaPro NanoStar from WyattWyatt Technology Corporation, the world leader in absolute macromolecular characterization instrumentation and software, publishes new application note highlighting a study made by Florida State University. Authored by Tsz Kin Martin Tsui, Claudius Mundoma and Hong Li from Florida State University, the paper showcases how the Wyatt DynaPro dynamic light scattering (DLS) system can be used to characterize homogeneity and stability of a heterodimeric protein complex (HPC) under various buffer conditions. The results demonstrate that the pH of the buffer solutions influences the sample homogeneity and stability.

A heterodimeric protein complex (HPC) from Pyrococcus furiosus is identified for its functional role in binding RNA. HPC has a total molecular weight of 65 kDa and has a tendency to form fibril materials in vitro as observed by electron microscopy. DLS data were acquired at 20°C using the Wyatt DynaPro NanoStar Dynamic Light Scattering (DLS) system. A total of 10 measurements were performed for each sample, and the stability and homogeneity determined from the polydispersity and autocorrelation functions using DLS provided information on the biophysical property of the HPC.

Hong Li, Department of Chemistry and Biochemistry and the Institute of Molecular Biophysics, Florida State University, said “Biological macromolecules are the focus of the Institute’s research – their form, function, interactions and mechanism of action. Challenging frontier areas include characterizations of the interactions between proteins and membranes, sugars or nucleic acids. The DynaPro NanoStar is ideally suited for studies of these complexes in addition to nanoparticles, vesicles, viruses and colloids. The results from the study demonstrate that the pH of the buffer solutions influences the sample homogeneity and stability, highlighting the conditions where polymerization of HPC is thermodynamically more favorable.

The Institute of Molecular Biophysics has a long history as a unique interdisciplinary research unit dedicated to the application of physical and chemical principles to the investigation of biological phenomena. Dr. Claudius Mundoma is the Director of the Physical Biochemistry facility which houses a broad array of biophysical technology used for macromolecular characterization of structure of macromolecules. He comments, “a technique such as Dynamic Light scattering is very efficient, requiring less time and sample than other techniques to determine particle size distribution and aggregation states of molecules.â€


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events