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HPLC Analysis of 6 Active Components of Caulis Lonicerae

Thermo Fisher Scientific pplication Note 299Thermo Fisher Scientific Inc. is pleased to announce an efficient, comprehensive HPLC QC method using an Acclaim Phenyl-1 Column for the separation of all six main active components of Caulis Lonicerae, an important traditional Chinese medicine. To regulate this natural treatment used for acute fever, headache, respiratory infection, and epidemic diseases, the Pharmacopoeia of the People's Republic of China (PPRC) requires two different methods, which combined only identify two main target components.

Application Note 299: HPLC Analysis of Six Active Components of Caulis Lonicerae Using a Phenyl-1 Column details the baseline resolution of loganin, sweroside, chlorogenic acid, caffeic acid, rutin, and galuteolin of the dried rattan of Lonicera japonica (Caprifoliaceae or honeysuckle family). Other HPLC methods that can identify the active components require long separation times and have insufficient peak resolution.

This application and many others can be found at under the Documents tab.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $12 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and UnityTM Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity.

All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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