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Informative ChIP Applications Compendium

publication date: Nov 7, 2016
 | 
author/source: Porvair Sciences Limited

Chromatrap® has published a comprehensive Applications Compendium that includes hints and tips to improve your Chromatin Immunoprecipitation (ChIP) experiments, increase the effectiveness of your ChIP-seq library preparation and brings together its latest application notes in one informative document.

Applications CompendiumChromatrap® solid-state ChIP technology has been adopted and shown by leading research groups worldwide to be more efficient than conventional bead-based methodologies. This is because the solid phase porous polymer, functionalized with either protein A or G, provides a greater surface area for chromatin antibody binding with very low non-specific binding. In addition, it uses a spin column approach, offering significant speed, process and carry-over advantages over sepharose or magnetic beads. DNA pull down with Chromatrap® is up to 25 times more than conventional methods, whilst the signal to noise ratio for DNA enrichment is 2 to 3 times better, even with low chromatin samples.

Chromatrap® ChIP assays are now available for classical qPCR, sequencing and ChIP from formaldehyde fixed paraffin embedded (FFPE) samples using either spin columns or 96-well filter plates. 

Chromatrap® is a business unit of Porvair Sciences, a wholly owned subsidiary of Porvair plc.


download a copy of the Applications Compendium


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