Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Brochures & Literature

 

 

View Channel

 

Conferences | Events

New Technology Enables Comprehensive Biopharmaceutical Characterization Workflow with a Single Platform

publication date: Jul 11, 2016
 | 
author/source: Thermo Fisher Scientific

Thermo Scientific Q Exactive BioPharma enables three powerful new protein characterization workflows on one instrument

thermo fisher scientificResearchers performing biopharmaceutical characterization can now benefit from a single system from Thermo Fisher Scientific that enables three powerful workflows for the complete characterization of biotherapeutics.

The new Thermo Scientific Q Exactive BioPharma MS/MS Hybrid Quadropole-Orbitrap mass spectrometer takes full advantage of the high-resolution, accurate-mass (HRAM) capabilities of the Orbitrap mass analyzer to enable three key protein characterization workflows; denatured and native MS intact analysis, subunit and top/middle-down analysis, and peptide mapping. Combined with recommended sample preparation and analysis methods based on Thermo Scientific reagents, columns, UHPLC and data processing technologies, the Q Exactive BioPharma constitutes a complete solution for the rigorous structural characterization of biotherapeutic compounds.

“Researchers require multiple MS techniques to perform the experiments essential for biopharmaceutical structure characterization,” said Jonathan Josephs, director, pharma/biopharma marketing, life sciences mass spectrometry at Thermo Fisher. “We now offer a single instrument that encompasses all of our leading technologies into one system, which can be used to analyze antibody-based biologics at the intact, subunit, and peptide levels—ultimately providing an efficient route to high quality results.”

The Thermo Scientific Q Exactive BioPharma features a new High Mass Range (HMR) mode which adds the ability for exceptional intact monoclonal antibody and antibody-drug conjugate (ADC) analysis, under both native intact and denatured intact conditions, to the existing capabilities for subunit top/middle-down and peptide mapping analysis.

When combined with Thermo Scientific BioPharma Finder Software, the technology is a powerful solution for biotherapeutic characterization. The integrated workflow-driven interface enables automated processing of data from peptide, subunit, and intact protein analyses. BioPharma Finder uses two deconvolution algorithms to take full advantage of the ultra-high-quality, high-resolution accurate mass (HRAM) data produced by Orbitrap-based mass spectrometers to provide superb accuracy for intact mass measurements. This powerful software can also be used to accurately characterize disulfide bond linkages, detect low-level impurities and automate the relative quantitation and reporting of sequence variants and post-translational modifications.

To further complete the solution, the new Thermo Scientific Vanquish Flex Binary UHPLC system   adds a binary solvent delivery option in the 1000 bar (15,000 psi) performance range. The system is built for high-speed, fast gradient applications and the Vanquish platform’s inherent outstanding retention time reproducibility. The Vanquish Flex Binary UHPLC system is the solution for pharmaceutical and biopharmaceutical laboratories performing qualitative and quantitative LC or LC-MS analysis. Ease-of-use and innovative technologies that are robust by design are making UHPLC widely accessible and enable easy and effective method transfer from established systems and applications.


more about thermo fisher scientific


more news from thermo fisher scientific


 

 

 


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners