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Recombinant Protein Fermentation Monitoring in Real Time

DionexDionex is proud to announce a customer-developed method that enables real-time monitoring of laboratory-scale protein expression. Until now, real-time monitoring of protein expression has proven difficult, due to the requirement for time-consuming cell harvesting, protein extraction, and purification procedures prior to analysis. Customer Application Note 110: Rapid Analysis of Recombinant Protein Production During Fermentation Using Dionex ProPac SCX Columns shows how the ProPac® SCX-10 column can be used for real-time monitoring of recombinant protein production, with reduced analysis time and smaller sample volume requirements. Further time savings can be achieved using the ProSwift® SCX-1S column.

This customer application note explains how to purify and analyze recombinant lysostaphin directly from cell-free lysate without any additional purification steps; this approach is advantageous as it permits rapid monitoring as the fermentation progresses. This method also demonstrates that small volumes of culture (as little as 1 mL) can provide sufficient material for rapid analysis.

This customer application note and many others can be found at under the Documents tab.

Dionex (NASDAQ:DNEX) is a global leader in the manufacturing and marketing of liquid chromatography and sample preparation systems, consumables, and software for chemical analysis. The company's systems are used worldwide in environmental analysis and by the life sciences, chemical, petrochemical, food and beverage, power generation, and electronics industries. Our expertise in applications and instrumentation helps analytical scientists to evaluate and develop pharmaceuticals, establish environmental regulations, and produce better industrial products.

ProPac and ProSwift are registered trademarks of Dionex Corporation.

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