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Quick and easy HPLC method for cannabidiol and melatonin separation

publication date: Jul 23, 2020
author/source: YMC Europe GmbH


Are you working with cannabinoids in medicinal products?

This application note describes how to obtain a fast separation of cannabidiol and melatonin which are compounds that can be used in medication for sleep disorders.
Excellent peak shapes are achieved using YMC-Triart C18 in less than 7 min.

Cannabidiol (CBD) is a non-psychoactive cannabinoid which has an easing, anti-inflammatory and antianxiety effect and is used to ease nausea. Melatonin is a hormone from serotonin and controls the day-night rhythm. For this reason, an American customer was interested in developing a sleeping aid containing both ingredients. Therefore, YMC was asked to develop a simplified method for the quanti-tative analysis of CBD and melatonin at a ratio of 5:1.

Due to its versatile column chemistry YMC-Triart C18 columns are the perfect starting point for method development.

The high lot-to-lot reproducibility of YMC-Triart C18 will ensure accurate results which is important for quality control.

This application note shows how to obtain a fast separation of CBD and melatonin with excellent peak shapes. The analysis is performed in only 7 minutes under gradient conditions using an YMC-Triart C18 HPLC column with 3 μm particles. The sample was prepared from 1 mg/mL stock solutions of melatonin (diluted in acetonitrile/water 45/55) and CBD (in ace-tonitrile) at concentrations of 0.05 mg/mL CBD and 0.01 mg/mL melatonin.

Get your copy of the application note now.


View App Note

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