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Thermo Fisher Scientific has developed an on-line solid-phase extraction–high-performance liquid chromatography (SPE-HPLC) method combined with ultraviolet (UV) absorbance detection for the simple and sensitive determination of tigecycline in a cell lysate sample.

Application Note 1074:Determination of Tigecycline in a Cell Lysate demonstrates that this approach, performed on a rapid separation LC (RSLC) system controlled by data management software, detects trace amounts of tigecycline in tigecycline-treated cells, concentrations so low that they cannot be assayed using a routine HPLC-UV detection method.

The prevalence of multidrug-resistant bacteria in hospitals underscores the need for new antibiotics to combat resistant pathogens. Tigecycline, a novel broad-spectrum antibiotic, exhibits activity against bacteria resistant to other antibiotic classes and resists deactivation by most known tetracycline-resistance mechanisms. But because tigecycline is a newly commercially available antibiotic, there are few HPLC-UV detection methods to quantify it in various biological tissues and fluids. Most other available detection methods are either too complex or lack the required sensitivity.

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