Follow us...

 

Search News Archives

Monthly Newsletter

 

Lab Bulletin May Newsletter

view the latest issue

Subscribe

News Channels

 

New Laboratory Products

 

Lab News

 

Microscopy & Image Analysis

 

Separation Science

 

Research & Case Studies

 

Literature

 

Videos

 

Events | Webinars

 

 

 

Conferences | Events

Analyzing intracellular molecular diffusion

Diffusion measurementOlympus has today introduced the new Diffusion Measurement module for Olympus ASW 2.1 software. Designed specifically for use with the Olympus FluoView FV1000 confocal microscope system for live cell imaging, this new software module provides users with the flexibility to undertake three different types of diffusion study: point fluorescence correlation spectroscopy; raster scan image correlation spectroscopy (RICS) and fluorescence recovery after photobleaching (FRAP). As a result, the Diffusion Measurement Package is ideal for a broad range of cell biology and biophysics applications. 

Intracellular diffusion is frequently analysed to assess the movements, interactions and microenvironments of intracellular molecules to determine the patterns and rates of molecular movement. In order to meet this need, the Olympus Diffusion Measurement Package enables researchers to execute three different types of diffusion study:

Point Fluorescence Correlation Spectroscopy

Data is obtained from a single location using point scanning, enabling fluctuations in fluorescence intensity to be recorded. The number of moving particles within the confocal volume is therefore easily calculated. With its high temporal and spatial resolution, this technique provides data on the diffusion constant or number of molecules, as well as the capability to analyse cross correlations between different molecules.

RICS

With the ability to provide diffusion measurements in various cellular regions, the software is able to present the user with a spatial mapping of diffusion time constants. As a result, users can easily define small regions of interest and subsequently create a diffusion map from a series of 2D images. A wide range of intracellular structures, from molecules in solution to cell membrane proteins, can therefore be accurately measured. Resulting data can be expressed as either a diffusion constant or the number of available molecules.

 FRAP

As a widely used fluorescence analysis technique, researchers commonly use FRAP to analyse the diffusion constant in a particular region of the cell, as molecules diffuse into the area after photobleaching.

The Diffusion Measurement Package enables users of the Olympus FluoView FV1000 to control and measure the diffusion constant with ease.

For further information please visit www.microscopy.olympus.eu


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners