Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Brochures & Literature

 

 

View Channel

 

Conferences | Events

Dolomite Bio Offers Single-Cell RNA-Seq to Tackle Kidney Research

publication date: Oct 12, 2017
 | 
author/source: Dolomite

MarkZiemann

Scientists at the Epigenetics in Human Health and Disease Laboratory, Monash University, Australia, have adopted Dolomite Bio’s Single Cell RNA-Seq System to further their research into animal models of diabetic nephropathy.

Mark Ziemann, research fellow and next generation sequencing specialist, explained: “Previously, we’ve run bulk RNA sequencing on these animal models, and discovered a lot of variability coming from different cell types in the kidney. Single cell RNA-Seq will help us disentangle the major cell types involved in the disease from those which are not, and determine which cells interact with the immune system. Given that this will be an ongoing focus of our research, we thought we’d invest in our own instrument for the benefit of the 65-strong department.”

“We chose Dolomite Bio’s system because it’s open and flexible, offering us the possibility of looking at epigenetic modifications – such as single-cell chromatin immunoprecipitation sequencing and DNA methylation analysis – in the future. In addition, other systems we considered turned out to be a lot more expensive on a per sample basis. We considered developing our own in-house set-up, but committing to reliable chips and pumps from a single supplier ensures that there will be fewer problems down the line, and there’s always someone on the other end of a phone to help if necessary.”

“A member of the Dolomite Bio team did a wonderful job providing training and installing the system in June and, by early August, we were already running samples. A recent species mixing experiment – sequenced on the Illumina MiSeq – enabled us to distinguish between human and mouse cells with very few doublets, which is encouraging. We already have a number of tissue analysis experiments lined up, and our system is working well.”


more about dolomite


more news from dolomite


 

 

 


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners