Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Brochures & Literature

 

 

View Channel

 

Conferences | Events

JPK’s NanoWizard AFM and CellHesion Systems are Used to Shed Light on the Development and Regeneration of the Central Nervous System in the Franze Group at the University of Cambridge

publication date: Oct 10, 2016
 | 
author/source: JPK Instruments AG

Dr Isabell Weber and Joy Thompson from the Franze Group with their JPK NanoWizard® system

JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports on the use of their NanoWizard® AFM and CellHesion® systems in the Department of Physiology, Development & Neuroscience at the University of Cambridge. 

Dr Kristian Franze is a lecturer in the Department of Physiology, Development and Neuroscience at the University of Cambridge. His major goal is to understand when, where and how mechanical signals, such as forces and local tissue stiffness, are involved in controlling cell development and function in the nervous system. With their JPK systems, they particularly want to figure out how the mechanical environment of cells in the central nervous system (CNS) looks like in the developing embryo as well as in regenerating spinal cord and brain tissue. Using AFM and CellHesion® techniques among other microscopies, both commercial and custom-made, Dr Franze and others have shown that nervous tissue is mechanically heterogeneous. Furthermore, they found that neurons constantly exert forces on their environment and that both neurons and glial cells respond to mechanical cues. Understanding how and when CNS cells actively exert forces and respond to their mechanical environment will shed new light on CNS development and could eventually lead to novel biomedical approaches to treat or circumvent pathologies that involve mechanical signaling. 

While AFM is well-accepted, the addition of a CellHesion® module greatly increases the force measurement capabilities. It provides the quantification of single cell-cell and cell-surface interactions under physiological conditions. In parallel to these forces, cytomechanical characteristics including stiffness and elasticity can be determined. In Dr Franze's group, the CellHesion® setup enables the measurement, for example, of brain tissue stiffness in living frog embryos with cellular resolution and the simultaneous observation of how fluorescently labelled neurons grow in that region. 

Dr Franze has been using NanoWizard® AFMs from JPK since 2002 when he bought his first system as a PhD student. He has continued to select systems from JPK ever since. Discussing his reasons for choosing systems from JPK, he says, “I very much appreciate the long working distance in z of 100 µm. This is key for the raster measurements of whole tissues in living embryos. I also like the ability of the Experiment PlannerTM which allows us to easily design our own experiments. The support we get from JPK's Alex Winkel is quite superb and underscores why we have such a great relationship with the company.” 

2016 has been a good year for publication of the group's research. Amongst others, they have had a paper in Nature Neuroscience1 (which contains the in vivo brain mechanics measurements). 


more about JPK instruments


more news from JPK instruments


 


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners