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Determination of Chloramphenicol in eggs by XLC-MS using the Symbiosis Pharma System

SymbiosisTM Pharma is Spark Holland's unique solution for integrated online SPE-LC-MS automation (XLC-MS). The system offers large flexibility in processing different types of samples selecting one of the three fully automated operational modes LC-MS; XLC-MS; AMD (Advanced Method Development). This application shows a fast method development for Chloramphenicol with the use of the AMD mode of Symbiosis Pharma together with the HySphereTM method development cartridge tray (Spark p.n.0722.650).

Chloramphenicol was originally isolated from a species of Streptomyces bacteria. Its antibiotic activity results from its interference with protein synthesis in invading microbes. However, it is a very toxic substance, its most serious and potentially lethal effect being depression of red blood cell production in bone marrow; cases of leukemia were also attributed to early use of hloramphenicol. Because of its toxicity, Chloramphenicol is rarely prescribed for infections that can be treated by other antibiotics.

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