Follow us...

 

Search News Archives

Channels

News

 

 

View Channel

Laboratory Products

 

 

View Channel

Special Offers and Promotions

 

Microscopy | Image Analysis

 

 

View Channel

Separation Science

 

 

View Channel

Coronavirus (COVID-19)

 

 

View Channel

Research & Case Studies

 

 

View Channel

Brochures & Literature

 

 

View Channel

 

Conferences | Events

Tailored Solutions for Exosome-related Research

publication date: Aug 25, 2020
 | 
author/source: AMS Biotechnology (Europe) Ltd

tailored-solutions-exosomerelated-research


AMSBIO offers a comprehensive range of end-to-end services for exosome purification, characterization, and engineering.

Exosomes are small endosome derived lipid nanoparticles, actively secreted by exocytosis in most living cells. Acting on tissues in both the local environment and further afield, they function as important signal carriers through their transport of RNA, proteins, lipids and DNA. Exosomes are involved in a wide range of normal and pathogenic processes, making exosome research crucial in multiple areas. Exosomes are a type of extracellular vesicle (EV) that show promise for use as unique drug carriers to deliver protease inhibitors to treat COVID-19. In this application, EVs may provide targeted delivery of protease inhibitors, with fewer systemic side effects. More importantly, EVs are highly suitable for major aseptic processing and can be upscaled for mass production. Currently, the FDA is backing applications to treat COVID-19, which provides a very good chance to use EVs to contribute to this vital area of research and development.

The Exosome Isolation & Quantification Service from AMSBIO provides reliable and reproducible isolation of exosomes from almost any biofluid ranging from plasma and tissue culture media to cerebrospinal fluid. Using this service, exosomes are isolated by ultracentrifugation and quality assessed using a NanoDrop spectrophotometer. Options are also available to isolate by differential/gradient ultracentrifugation, immunoaffinity, size exclusion chromatography, precipitation/low speed centrifugation.

For scientists interested in exosome miRNA isolation and sequencing - AMSBIO offer a service to extract RNA from exosomes, and subject it to the Small RNA-Sequencing. In addition to basic analysis will be performed on the raw data, screening for specific miRNA can also be performed.

AMSBIO also offers analysis of exosome surface proteins using MACSPlex kit. For proteomics analysis, samples will be analyzed by LC/Q-TOF mass spectrometry to characterize the peptides formed. The analyzed samples are also differentially analyzed using molecular features finder and mass profiler to determine changes in the peptide profile. The resultant MS/MS data from the selected peptides will be imported into Spectrum Mill proteomic server software to identify the protein that is consistent with the imported peptide sequence. Both NCBInr and Swiss-Prot databases are available to search for users of this service.

In addition, AMSBIO can undertake a wide range of exosome functional assays including exosome-induced cell proliferation, exosome induced collagen production, Exosome-induced inhibition of inflammatory cytokines and In vitro wound healing (scratch assay).

 

Click here to learn more

 

About AMS Biotechnology

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognised as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cell and cell therapy applications these include high quality solutions for viral delivery (lentivirus, adenovirus and adeno-associated virus) in addition to GMP cryopreservation technology.

 

more news from AMSBIO



If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners