Follow us on
Search News Archives
Conferences | Events
Monthly Newsletter
Lab Bulletin Media Partners

Glass columns – not only for biochromatography

In the laboratory glass columns are favoured by many chromatographers because of their inertness towards proteins. Also the possibility to clean the packed columns with highly alkaline caustic soda solutions, a widely-used purification step, is a good argument for glass columns.

While the suitability of glass columns for biochromatography is well-known, the use in NP- or RP-chromatography with silica based stationary phases is rarely considered. The main reason maybe the concern that the high back pressure of 15μm or smaller silica particles may exceed the specifications of the column. However glass columns that withstand high back pressures from 30 (for 50mm i.d) up to 80bar (for 5mm i.d.) are available. These allow most demanding separations with long silica chromatographic beds or high flow rates for fast elution.

The figure shows the separation of a standard sample, using different flow rates, to illustrate the enormous time saving of 75% that is made possible by these greater pressure ratings.

Kronlab Glass Columns


Column:Kronlab EcoPLUS 125 x 25 mm i.d.
Packed with YMC ODS-AQ 10µm
Mobile Phase:Acetonitril / water (50:50)
Injection-Vol.:500 µl
Detection:254 nm
 Uracil0,175 mg/ml
 Methyl benzoate1,25 µl/ml
 Naphthalene0,5 mg/ml
 Butyl benzoate4,0 µl/ml

For further information please contact YMC Europe GmbH, Dr. Wolfgang Schräder, Phone: +49 (0)2064 427-290, Fax: +49 (0)2064 427-222, or visit

If you have not logged into the website then please enter your details below.

Can't find what you are looking for?

Search by company, product or location

Company Name:



Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have a product enquiry, please contact the supplier directly.

Lab Bulletin is published by newleaf marketing communications ltd