Search News Archives
Follow us on
Conferences | Events

Media Partners


Glass columns – not only for biochromatography

In the laboratory glass columns are favoured by many chromatographers because of their inertness towards proteins. Also the possibility to clean the packed columns with highly alkaline caustic soda solutions, a widely-used purification step, is a good argument for glass columns.

While the suitability of glass columns for biochromatography is well-known, the use in NP- or RP-chromatography with silica based stationary phases is rarely considered. The main reason maybe the concern that the high back pressure of 15μm or smaller silica particles may exceed the specifications of the column. However glass columns that withstand high back pressures from 30 (for 50mm i.d) up to 80bar (for 5mm i.d.) are available. These allow most demanding separations with long silica chromatographic beds or high flow rates for fast elution.

The figure shows the separation of a standard sample, using different flow rates, to illustrate the enormous time saving of 75% that is made possible by these greater pressure ratings.

Kronlab Glass Columns


Column:Kronlab EcoPLUS 125 x 25 mm i.d.
Packed with YMC ODS-AQ 10µm
Mobile Phase:Acetonitril / water (50:50)
Injection-Vol.:500 µl
Detection:254 nm
 Uracil0,175 mg/ml
 Methyl benzoate1,25 µl/ml
 Naphthalene0,5 mg/ml
 Butyl benzoate4,0 µl/ml

For further information please contact YMC Europe GmbH, Dr. Wolfgang Schräder, Phone: +49 (0)2064 427-290, Fax: +49 (0)2064 427-222, or visit

If you have not logged into the website then please enter your details below.


Popular this month



Renishaw Technology Used to Identify Microplastics in the Environment


Cell culture made easy with new 24-channel pipetting heads for VIAFLO 96/384


You spoke, we listened – meet ASSIST PLUS


Tomocube 3D Microscope Captures Key Cellular Dynamic Responses to Parkinson Drug Treatment


Nine top companies awarded Pittcon 2019 Excellence Awards


New Gilson Benchtop Instruments, The Perfect Complement to Your Workflow


World’s First Voice-Powered Digital Assistant for Scientists


Pesticide Standard Mixtures for Cannabis Testing


Gene Editing Automation Collaboration


Extended spectrum of applications for centrifugation




Can't find what you are looking for?

Search by company or by product

Company Name:


Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd