Follow us...


Search News Archives

Special Offers and Promotions



News Channels

Lab News


View Channel

New Laboratory Products


View Channel

Microscopy | Image Analysis


View Channel

Separation Science


View Channel

Research & Case Studies


View Channel

Coronavirus (COVID-19)



View Channel

Brochures & Literature


View Channel



Conferences | Events

New UV Shadowing Application of G:BOX Image Analyser Is Providing Accurate, Safe Analysis of DNA without using Labels

G:BOX image system Syngene, a world-leading manufacturer of image analysis solutions, is delighted to announce its G:BOX image system is being used at the University of Southampton for a UV shadowing technique to detect unlabelled DNA, enabling scientists there to safely analyse a range of different oligonucleotides. 

The G:BOX is being used by researchers in the School of Chemistry at the University of Southampton to visualise oligonucleotides in an acrylamide gel, placed on a silica-coated TLC plate. The G:BOX illuminates the oligonucleotides by shining UV light (254nm) on to the gel. The oligonucleotides cast a shadow against the TLC plate's fluorescent background, allowing visualisation of the DNA bands with the G:BOX's high resolution CCD camera. The image of the UV shadow is captured using the G:BOX's capture software and the bands are then analysed with GeneTools software to automatically calculate the size and molecular weight of the DNA.   

Dr Afaf El-Sagheer, a Research Fellow in the School of Chemistry commented: "We are synthesising different oligonucleotides using a variety of different organic reactions. We use UV shadowing to analyse them because it provides us with a clear impression of what is happening and is more convenient than using toxic stains like Ethidium bromide. We can detect as little as 0.1 OD260 of DNA and visualise DNA ranging from 2-300 bps using the G:BOX, which is sensitive enough for all our research needs."  

Laura Sullivan, Syngene's Divisional Manager, added: "We are excited to see the G:BOX being used successfully for UV shadowing. The research at the University of Southampton demonstrates that the G:BOX offers an accurate method of detecting oligonucleotides or small sections of DNA without the need to use mutagenic Ethidium bromide, hazardous radiolabels or expensive non-toxic fluorescent dyes.  For scientists with a limited consumables budget or those wanting to extract DNA from their gels, using the G:BOX image analysis system for UV shadowing provides the perfect solution."

For further information please visit

If you have not logged into the website then please enter your details below.



Popular this Month...

Our Top 10 most popular articles this month


Today's Picks...




Looking for a Supplier?

Search by company or by product


Company Name:




Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.



Media Partner