Thermo Fisher Scientific has introduced the Gibco™ Expi293™ PRO Expression System, a next-generation HEK293 transient expression system designed to deliver exceptional protein yields for a wider variety of proteins, including low-yield and challenging proteins, with higher throughput and speed.

HEK293 cells are the most commonly used mammalian cell host for protein expression. They are utilized in a variety of research applications, including drug discovery, structural biology, recombinant vaccines, and cell and gene therapies. For researchers working in these fields, producing increasingly complex proteins with higher yields and higher throughput is critical to accelerating breakthroughs in protein research and therapeutic drug development.

The Expi293 PRO Expression System offers exceptional yields, speeds and efficiencies for a wide range of proteins, enabling the expression of proteins that were previously unable to be expressed. 

Other current protein expression solutions on the market offer limited ability to produce high yields of traditionally challenging proteins, such as membrane proteins, intracellular proteins, secreted proteins, viral proteins and Fc fusion proteins. Many available solutions are also not optimized for scaling down into a 96-well plate format, which hinders adaptation into automated platforms, reducing throughput and scalability. Due to these challenges, researchers have often had to invest more time, money, labor, equipment, reagents and plasticware to achieve the yields necessary for advancing their research.

The Expi293 PRO Expression System is composed of a new, clonally derived HEK293 cell line, Gibco Expi293 PRO Cells, which can produce difficult-to-express proteins and support protein production on a milliliter to multi-liter scale. Additionally, the system includes Gibco Expi293 Medium, an optimized culture medium, and Gibco Expi293 PRO Transfection Kit, which enables high-density cell transfection. It also includes components and protocols designed for easy incorporation into automated workflows and a streamlined protocol to minimize hands-on time and maximize the number of transfections that can be performed per week.

Together, these optimized components enable the Expi293 PRO Expression System to produce significant improvements in protein yields across a broad range of proteins, compared to existing 293 expression systems, with protein titers of up to five grams per liter. The system also offers a more sustainable protein expression solution, featuring greener packaging and helping to reduce plastic consumption, reagent consumption and labor by at least 3-fold.

“Achieving sufficient yields of difficult-to-express proteins remains a major challenge for scientists, often slowing down critical research and drug discovery while increasing time, labor and cost,” said Tiffani Manolis, Vice President and General Manager, Cell Biology, Thermo Fisher Scientific. “Built on the trusted legacy of our Expi293 products, the Gibco Expi293 PRO Expression System was developed to meet evolving demands, from high-throughput screening in 96-well plates to liter-scale production. As protein targets grow more complex, researchers need a system that offers both flexibility and efficiency across diverse protein classes. Expi293 PRO helps overcome expression bottlenecks, accelerating scientific progress and ultimately improving global health outcomes.”

“The Expi293 PRO system saved our COVID-19 response effort and has since enabled success in previously shelved projects,” said Dominic Esposito, Ph.D., Frederick National Laboratory for Cancer Research. “It’s the most flexible and effective HEK293 expression system I’ve used in my 25 years of mammalian protein expression.”

Key Applications

Scientists in the fields of protein research and drug development, looking to produce antibodies, membrane proteins, Fc fusion proteins, secreted proteins, viral proteins, intracellular proteins or any other challenging or low-expressing proteins.

 
Features/Benefits

• Access to new protein targets: Offers researchers access to new protein targets by overcoming current challenges in expressing complex proteins with low or no yield.
• Exceptional protein yields: Delivers a significant increase in protein yields, up to five grams per liter.
• Cost-effective solution: Produces higher protein yields at the lowest cost per milligram compared to other protein expression systems.
• Increased efficiency: Produces proteins in less time with fewer steps and delivers protein in as little as two to four days.
• High throughput and automation compatible: Includes simplified protocols and optimized reagents that seamlessly integrate with small-scale and automated platforms.
• Improved sustainability: Reduces reagents, plastic consumables and labor by three times, and uses fewer shipping materials per milligram of protein than other comparable protein expression systems.
   

*FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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