Channels

 

Special Offers & Promotions

 

 

Latest News

 

 

View Channel

New Products

 

 

View Channel

Video Presentations

 

 

View Channel

Separation Science

 

 

View Channel

Microscopy & Image Analysis

 

 

View Channel

Laboratory Automation & IT Solutions

 

 

View Channel

 

A Robust, Quantitative Alternative to the Scratch Assay

Oris™ Cell Migration Assay New independent research demonstrates that the OrisTM Cell Migration Assay presents a superior quantitative, high throughput alternative to the scratch assay.

Cell migration is integral to many physiological processes, including embryonic development, tissue regeneration, and wound healing.  One assay commonly used to study cell migration is the scratch assay creating a cell-free gap, or "scratch", on a confluent cell monolayer. Whilst this method is popular, the inherent variability in how scratches are produced and physical damage to cells make it difficult to obtain quantitative and statistically valid measurements of cell migration. Furthermore it has been demonstrated that the "scratch" will damage any underlying extracellular matrix (ECM).

The OrisTM Cell Migration Assay has been shown, in comparative testing (1,2), to offer a superior alternative to the scratch assay, permitting formation of precisely placed and homogeneously sized cell-free areas into which migration can occur without releasing factors from wounded or dead cells.

During cell seeding, OrisTM Assays use silicon stoppers or a non-toxic biocompatible gel (BCG) to form a centrally located and temporary cell-free zone on cell culture surfaces in a 96 or 384-wells format. After removal of the stoppers or dissolution of the BCG, the migration of cells into the detection zone can be observed either in real time or after further experimental processing. This breakthrough product enables researchers to capture and quantify cell migration data while substantially improving assay efficiency and dramatically reducing assay costs.

Measurement of cell migration enables researchers to better understand the impact of candidate therapeutic compounds on biological pathways and cellular behavior. The OrisTM cell migration platform offers a robust and statistically validated assay capable of supporting quantitative compound evaluation using a single determination per data point. Available from AMSBIO - ORISTM Assay kits support quantitative analysis of compound structure-activity relationships and provide reliable and reproducible measures of compound potency either in medium or high throughput formats.

Further information on OrisTM Assay Kits is available at www.amsbio.com/Oris-Cell-Migration-and-Invasion-Assays.aspx or by contacting AMSBIO on tel. +44-1235-828200 or email info@amsbio.com.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized as a leading international provider of unique & innovative products & custom services for life sciences research. The amsbio range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, electrophoresis, glycobiology, posttranslational modification & stem cell biology.

References    
1. Journal of Biomolecular Screening 2011, 16, pp155-163 
2. http://www.amsbio.com/brochures/Oris_Scratch_Assay_Comparison.pdf


If you have not logged into the website then please enter your details below.



 

Subscribe to any of our newsletters for the latest on new laboratory products, industry news, case studies and much more!

Newsletters from Lab Bulletin

 

Request your free copies HERE

 

 

 

Popular this Month

Top 10 most popular articles this month

 

 

Today's Picks

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd.


 

Media Partners

 

Exhibitions & Events