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Reporter Lentivirus for Signaling Pathway Studies

publication date: Jun 6, 2017
 | 
author/source: AMSBIO

AMSBIO has introduced a new range of ready-to-use Reporter Lentivirus for assessing cell signaling activities in virtually any mammalian cell type. 

Reporter LentivirusThese pre-made products use a unique combination of transcription factor reporter technology coupled with lentiviral delivery to provide a simple, highly sensitive method capable of operating over a large dynamic range.

By measuring the activities of a reporter, AMSBIO Reporter Lentivirus can be used for performing gene regulation studies in living mammalian cells and for functional genomics as well as drug screening cell signaling assays.  In addition Reporter Lentivirus can be used for generating your own pathway screening assay cell lines for the cell based assay in your desired cell types.

AMSBIO offers a wide range of “signaling pathway lentiviruses” for rapid, sensitive and quantitative monitoring of transduction pathway activation. These lentiviruses express a luminescent or fluorescent reporter under a pathway specific promoter or under a minimal promoter with embedded multiple tandem repeats incorporating pathway specific transcription factor binding motif. The pathway null control lentivirus has the minimal promoter without any signal pathway’s response elements inside the promoter. These lentiviruses also express an antibiotic selection marker or another fluorescent marker under a constitutive RSV promoter which serve as the internal normalization control or as a convenient monitoring method for virus transduction efficiency. Options include androgen and estrogen receptors, JNK, NF-?B , Notch and Wnt signaling pathways.


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