Follow us...

 

Search News Archives

News Channels

 

New Laboratory Products

Lab News

What's going on at Lab Innovations

Research & Case Studies

Microscopy | Image Analysis

Separation Science

Brochures & Literature

Videos

Events | Webinars

 

 

Conferences | Events

All options covered with Lab M’s C. sakazakii media range

Commonly found in the environment and able to survive dessication, opportunistic pathogen Cronobacter sakazakii is known to pose a risk in the production of dried milk products. Since the organism is associated with sporadic cases and outbreaks of meningitis, necrotising enterocolitis and sepsis, especially in newborns, its reliable detection and monitoring crucial. In developing specific HarlequinTM chromogenic media, supported by a selective enrichment broth, Lab M provides the three key elements which ensure all current C. sakazakii testing protocols are covered: CSEB - Cronobacter sakazakii Enrichment Broth (ISO); HarlequinTM CSIM - Cronobacter sakazakii Isolation Medium (ISO); and HarlequinTM CSA-DFI - Cronobacter sakazakii Agar - DFI Formulation.

 

CSEB (ISO) is based on lauryl sulphate tryptose broth and is used for the secondary enrichment of C. sakazakii. Extra sodium chloride included in the medium delivers enhanced selectivity against competing organisms, while vancomycin inhibits the growth of Gram-positive bacteria.

 

The chromogenic medium HarlequinTM CSIM (ISO) is designed for the isolation of C. sakazakii from milk and milk products. Alpha-glucosidase, expressed by C. sakazakii, hydrolyses the chromogenic substrate in the medium, producing green to blue-green colonies. Other Enterobacteriaceae appear colourless, or purple as a result of crystal violet uptake from the medium. The combination of sodium desoxycholate, crystal violet and an elevated incubation temperature ensure selectivity.

 

Harlequin CSA-DFI also uses alpha-glucosidase activity to differentiate C. sakazakii strains from other members of the Enterobacteriaceae. The production of alpha-glucosidase by the pathogen results in hydrolysis of the chromogenic substrate and the formation of green colonies, with other Enterobacteriaceae appearing colourless. Hydrogen sulphide-producing organisms such as Salmonella and Proteus species appear grey, brown or black. 

 

C. sakazakii is associated with sporadic cases and outbreaks of meningitis, necrotising enterocolitis and sepsis, especially in newborns. 

 

To request a sample email info@labm.com or for further information please visit www.labm.com  


If you have not logged into the website then please enter your details below.



 

 

Popular this Month...

Our Top 10 most popular articles this month

 

Today's Picks...

 

 


 

Looking for a Supplier?

Search by company or by product

 


Company Name:

Product:


 

 

Please note Lab Bulletin does not sell, supply any of the products featured on this website. If you have an enquiry, please use the contact form below the article or company profile and we will send your request to the supplier so that they can contact you directly.

Lab Bulletin is published by newleaf marketing communications ltd

 


Promotions

 

Media Partners