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Phenomenex Online Video Shows How to Maximize HPLC Results with High-Performance Media

Smaller diameter connective tubing minimizes HPLC system dead volumePhenomenex Inc., a global leader in the research and manufacture of advanced technologies for the separation sciences, has produced an online video tutorial on optimizing conventional HPLC systems for use with advanced, high-performance media.  New core-shell technology columns, such as Kinetex® from Phenomenex, can produce ultra-high performance results on standard LC systems.  Extra dead volume in HPLC instruments prevents maximum performance of the new, high-performance columns.  With easy HPLC system modifications, Kinetex users can realize performance improvements of up to 130 percent.  The Phenomenex video tutorial demonstrates simple ways to minimize this dead volume, such as using smaller-diameter connective tubing and switching to a low-volume needle seat and detector flow cell. 

"We developed and produced this tutorial to help chromatographers realize maximum potential from the new column technology on existing systems," commented Jeremy Bierman, brand manager for Phenomenex.  "Recent studies have shown that Kinetex core-shell columns are redefining what is possible in HPLC and that improvements to even the best instruments must be made before the full potential of these new high-performance columns is realized."

The video tutorial, which can be viewed at, shows step-by-step instructions that LC users can follow in their own labs, along with a case study on an Agilent 1100 system demonstrating the improved results that these optimization steps can produce.

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, clinical, government and academic laboratories.  From drug discovery and pharmaceutical development to disease diagnosis, food safety and environmental analysis, Phenomenex chromatography solutions accelerate science and help researchers improve global health and well-being.  For more information on Phenomenex, visit

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