In this application note the drug-to-antibody ratio (DAR) of a cys-linked ADC-mimic is determined using online native HIC-MS. Using the innovative BioPro HIC BF column and a direct coupling to the mass spectrometer even degraded payloads can be detected. The application also demonstrates how to overcome the high salt concentrations which are necessary for HIC methods....
Okadaic acid is diarrhetic toxin (DSP) which can be found in shellfish. Due to its inhibitory effects towards specific protein phosphatases it is a promising compound for various potential medical uses. This application note shows the LC-MS/MS analysis of okadaic acid and its derivates with different YMC-Triart phases. All phases are suitable for the separation of these compounds with high resolution....
In some methods derived from the Pharmacopoeia the stipulation of a specific injection solvent can lead to issues with peak shape and a reduced sensitivity for some or all compounds analysed. With a custom injection program, you can overcome this hurdle and still be conform to Pharmacopoeia guidelines. Your gain will be a higher chance to achieve your system suitability criterions and a more reproducible and robust analysis...
Are you looking for robust and reproducible columns to evaluate peptides? Do you want to separate oligonucleotides with high resolution? Robust YMC-Triart C18 columns are the perfect choice for reliable and reproducible (U)HPLC analyses of peptides and oligonucleotides with exceptional resolution. The wide temperature- (up to 90°C) and pH-range (1-12) provide increased flexibility....
In order to address separation requirements of different compounds, YMC offers two different HIC columns, BioPro HIC HT and BioPro HIC BF. They both have in common their non-porous polymethacrylate particle, while their properties and chromatographic behaviours are quite different. In this application note the retention behaviour of BioPro HIC HT and BioPro HIC BF columns is compared by separating two commercial monoclonal antibodies from each other.
(U)HPLC analysis of original composition of cannabis plant material is necessary for such purposes as phenotype determination and quality control of medical cannabis used for therapeutic treatment. For fast and secure results YMC developed robust (U)HPLC methods for RP and chiral LC. Also, challenging distinction between cannabigerol (CBG) andcannabidiol (CBD) are easily achieved....
If you are looking for a column to separate your antibodies from their fragments as well as from their aggregates, YMC-SEC MAB is your first choice! The column is designed to separate high and low molecular weight ranges, due to its 250 Å pore size. High column stability as well as excellent reproducibility is guaranteed! The 2019 SCIENTISTS’ CHOICE AWARD NOMINEE is designed for...
Are you working with cannabinoids in medicinal products? This application note describes how to obtain a fast separation of cannabidiol and melatonin which are compounds that can be used in medication for sleep disorders. Excellent peak shapes are achieved using YMC-Triart C18 in less than 7 min. Get your copy of the application note now....
Are you required to analyse very small sample amounts? Or do you need to analyse very low concentrations of antibodies in different kinds of matrices? By using of microLC-MS/MS, only a very small amount of valuable samples, e.g. solutions prepared for patient application (usually a dilution of the dosage form), is required to identify and quantify. In addition, microLC-MS/MS can also be used to monitor residues on surfaces or materials in hospitals, pharmacies or laboratories that prepare solutions for patient application....
You are looking for a state-of-the-art hydrophobic interaction chromatography column? With high resolution at high throughput, but also with superior batch-to-batch reproducibility? Then BioPro HIC HT is the ideal choice for you! YMC’s HIC column, BioPro HIC HT, is especially designed for analysis of biopharmaceuticals such as antibody-drug-conjugates (ADCs). BioPro HIC HT columns are the ideal choice for drug-to-antibody ratio (DAR) determination due to a novel and optimised surface chemistry....
Save time and order prepacked glass columns! Are you lacking the time to pack your column? Or don’t you have sufficient experience? Here is the solution! Rely on the experience of YMC and use prepacked ECO or ECOPLUS columns. Our packing expertise is your advantage. You may choose from the following packing services for ECO and ECOPLUS columns in standard and custom dimensions...
This application note shows a high-resolution UHPLC separation using a robust YMC-Triart C18 column including the previous sample preparation. The simultaneous analysis of etizolam, triazolam, and their metabolites 8-ethylhydroxyetizolam, alpha-hydroxyetizolam, 4-hydroxytriazolam and alpha-hydroxytriazolam in biological samples is performed by MS/MS detection....
Are you working with YMC’s classic columns such as YMC-Pack Pro C18, YMC-Pack ODS-A/AQ or YMC-Pack Polyamine II? Are you looking for specific information such as phase specifications etc for these columns? In that case the new YMC Classics eCatalogue is your perfect resource for this kind of data....
Versatile (U)HPLC-hybrid columns, now also in C8!
Nov 16, 2011With YMC-TriArt challenging pH-stability and high temperatures are no longer a limitation to your work. Above all due to its unique particle composition a balanced hydrophibicty and silanol activity are achieved which makes YMC-TriArt a â€śFirst Choiceâ€ť column in method development...
Are you sure your column has been packed the best possible way?
Jul 13, 2011Until now the extremely efficient YMC-BioPro IEX phases have only been available as bulk media. Still it was introduced into many screening procedures in industry, because of its high quality. Now it is even easier to evaluate its extremely good cost-efficiency ratio: YMC announces the introduction of pre-packed IEX columns...
The YMC HG-series: High Quality low price silica material
Apr 18, 2011Is it possible to get the best of both worlds? Better quality at better price? It is possible with the new YMC HG-grade series of silica based materials! These new phases combine the trusted selectivity of the YMC*gel materials with a new high performance silica base. The most obvious advantage of the new silica base is its extended mechanical stability which is due to an improved production process for the base silica...
Easy handling with Kronlab Pilot columns
Feb 22, 2011As a long-time specialist in the field of glass Pilot columns for lab and production separations, Kronlab specialists are continuously in close dialogue with chromatography end-users from Pharma/Biotech as well as research and education fields. This leads to numerous technical and practical modifications which make your every-day work a lot easier.
Preparative Chiral Purifications with YMC*Gel Chiral NEA
Oct 28, 2010Chirality has become vitally important in the production of pharmaceuticals, agrochemicals and related products, due to the dramatically different pharmacology the various optical isomers can present, ranging from no activity through undesirable effects to having potentially catastrophic and sometimes life threatening adverse effects. This has led to the development of highly efficient chiral stationary phases (CSP) for analytical and preparative scale separations
Pilot columns made to measure!
Oct 1, 2010It does not matter if itâ€™s tall or short, fat or thin - Pilot columns are increasingly being manufactured to customersâ€™ demands.
New brochure "YMC Phases for Biochromatography"
Sep 29, 2010The new HPLC brochure â€śYMC Phases for Biochromatographyâ€ť is available now! It contains current applications for biomolecules using various separation mechanisms including IEX, SEC, RP and HILIC.
Tricky syntheses in the micro-reactor
Jun 24, 2010YMC is a specialist in difficult, multistep syntheses in the micro-reactor. Higher yields, substances that are available more quickly and with extensive downstream processing guarantee substances up to the highest purity.
Invitation to YMCâ€™s free vendor lunch seminar at SPICA
Jun 24, 2010Every two years the leaders in preparative chromatography meet for the Symposium on Preparative and Industrial Chromatography and Allied Techniques (SPICA). As a supplier strongly rooted in preparative chromatography, YMC will participate in two ways:
Glass columns â€“ not only for biochromatography
Mar 24, 2010In the laboratory glass columns are favoured by many chromatographers because of their inertness towards proteins. Also the possibility to clean the packed columns with highly alkaline caustic soda solutions, a widely-used purification step, is a good argument for glass columns.
Binary prep. HPLC-system for processing large volumes of ureacontaining protein solutions
Mar 18, 2010Often client specific requirements for multipurpose equipment can not be solved with â€śoff the shelfâ€ś solutions. In this specific case, the Kronlab custom build approach proved very useful: every component has been specifically approved for the application at hand and following a specified procedure it is integrated into an LC system. The result is a made-to-measure system.
New YMC microreactor Systems â€“ Microreaction technology got potential
Mar 11, 2010Microreactor technology, is currently one of the most innovative techniques in the field of chemical synthesis and similar fields. Now specific microreactors are coming to laboratories. Scientific advances throughout the world have demonstrated the many advantages which can be achieved through the use of microreactors for chemical reactions and processes.
Binary Prep LC System for isolation of components from human blood serum
Mar 11, 2010Customised requirements for GMP-conforming extraction of substances are often not satisfied by â€śoff-the-shelfâ€ť solutions. Every component in the well-established modular systems from Kronlab meets the requirements which will be demanded by the customersâ€™ needs for an established procedure. The result: A made-to-measure system
Workshop: Preparative liquid chromatography
Jan 14, 2010Run at our laboratory facilities in Dinslaken, Germany, this hands-on course is designed to introduce both theory and practical concepts of preparative liquid chromatography.
HPLC-Tip: Degassing of the mobile phase
Dec 23, 2009A released gas in mobile phases during analysis causes noisy baselines and pressure fluctuations which affect sensitivity and reproducibility. It also severely compromises the use of fluorescence-, refractive-index- or UV-detectors (particularly at wavelengths below 210 nm). As a consequence effective mobile phase degassing must be employed for many analyses.
The Bio-Chromatographersâ€™ wish
Dec 23, 2009Simply high dynamic binding capacities (DBCâ€™s) are no longer sufficient. High DBC, at high flow rates with close to 100% recovery, across a wide range of molecular weights, this is what chromatographers look for in modern ion exchange resins. â€śBioProâ€ť YMCâ€™s hugely successful ion exchange material offers exactly this. The growing user base confirms very good separation, high dynamic binding capacities and excellent recoveries for the anion as well as the cation exchanger material.
New: YMC-Triart C18
Dec 23, 2009With YMC-Triart challenging pH-Stability and high temperatures are no longer a limitation to your work. Above all due to its unique particle composition a balanced hydrophibicty and silanol activity are achieved which makes YMC-Triart a â€śFirst Choiceâ€ť column in method development!
Preheating of mobile phases - "nice-to-have" or "need-to-have"?
Dec 23, 2009Controlling the temperature of a column is a prerequisite for reproducible results in HPLC. Column ovens are part of most HPLC systems and many of them offer space even for semi preparative columns. But with increasing inner diameter the heat transfer from the air surrounding the column to the whole cross section of the column (filled with the cold mobile phase) becomes more and more difficult. This may result in bad peak shapes, as the radial temperature gradientcauses different separation conditions for every part of the sample depending on its distance to the outer diameter.
As chromatographers, we all know the problems during method development - â€śWhich phase is suitable and allows a simple and robust separation?â€ť In the field of biochromatography, phase selection is a key to success!
Are you looking for a new ion exchange material? Have you tried the new BioPro material from YMC - it will give you a significant alternative.
Kronlab ECO glass columns
Dec 23, 2009Kronlab ECO glass columns meet highest quality demands. They are best suited for various chromatographic techniques like ion exchange, affinity and size exclusion chromatography.
Ultra Fast LC for Peptide mapping
Dec 23, 2009In order to succeed in developing protein-based drugs, a fast and easy detection of alteration within the protein structure is necessary. In many cases, peptide mapping is the method of choice, where proteins are analysed after digestion.
New: YMCâ€™s Applicationsbook for Ultra FastLC!
Dec 23, 2009The new YMC-UltraHT brochure from YMC includes information on how to transfer methods form existing, conventional methods to ultra fast LC, without generating excessive backpressure and without the need of specialised ultra high pressure chromatography equipment.
The method transfer from a conventional column to an ultra fast analysis for alkaloids has shown that the use of YMC-UltraHT 2 Âµm column and adjustment of flow parameters for the smaller column dimensions can reduce the analyses time by about 85% accompany with significant solvent savings and significant solvent savings whilst increasing the sample throughput in the laboratory.
HPLC hint: The advantage of premixed solvents in HPLC
Dec 23, 2009For several reasons the storage of 100% pure solvents in the reservoir bottles of an HPLC system can be a problem. Pure acetonitrile, in particular, affects the system by adsorption of polymers from transmission lines resulting in sticky surfaces that inhibit the opening and closing of the valves
Simultaneous separation of pesticides by LC/MS
Dec 23, 2009With the constantly rising population and frequent uncertain weather, the need for high yielding agriculture is paramount. This means that use of plant protection agents, such as herbicides and insecticides is necessary, in order to secure harvests.
Facing the challenge of efficiency in bio-pharmaceutical industry
Dec 23, 2009With continuously increasing volumetric productivity in fermentation processes, new stationary phases can help to overcome shortcomings in downstream processing. In order to address this YMC has developed a new Ion Exchange resin with high dynamic binding capacity and low nonspecific interaction. This helps to reduce â€ścost of goodsâ€ť in the production process.
FastLC Application for Curcumin
Dec 23, 2009The plant pigment curcumin, found in the popular Indian curry spice turmeric, is well known as an antioxidant for foods. However, researches have paid attention to another characteristic. Curcumin is highly active against the growth of certain cancers.
YMC is pleased to introduce Exphere, a new family of bulk packing materials, specifically designed for preparative liquid chromatography. Exphere combines YMC's novel high strength silica with a special polymeric surface modification technique.
Melamine in pet-food
Dec 23, 2009Melamine (sometimes confused with the plant and animal compound, melanine or another compound, melanin) is an industrial chemical used in the manufacture of plastic products. Despite that, tests showed that a number of milk companies have used it in processing milk to artificially increase the apparent protein content by addition of non-protein nitrogen and so allowing illegal dilution of the product with water with a corresponding increase in sales value.