This Application Note shows how to precisely analyse six oligonucleotides with optimum peak shapes and clear resolution. The ultra-short YMC Accura BioPro IEX QF column (30 mm) supports fast, routine workflows. Its thermal stability enables elevated temperatures of 70 °C providing improved peak shapes...

Analysis of PFAS in human serum using a YMC-Triart C18 column in combination with an online SPE column-switching method. A highly sensitive LC-MS/MS method for the analysis of 28 PFAS in human serum is demonstrated in this Application Note. By combining online SPE and the robust YMC-Triart C18 column, this setup ensures excellent separation, high throughput and reliable quantification...

This Application Note presents an optimised SEC-MS method for the high-performance separation and characterisation of a single-chain variable fragment (scFv) and its drug conjugates (FDC). Using a bioinert PEEK-lined YMC-Pack Diol-120 metal-free column combined with MS compatible conditions delivers sharp peaks & precise resolution, enhanced signal response and reliable results. Boost your analytical expertise – download the Application Note now!...

The demand for faster and more reliable cannabinoid analysis is growing with the expanding use of medicinal cannabis. Traditional HPLC methods often fall short in resolution due to the similar structures of cannabinoids. This application note presents a rapid UHPLC method using the YMC-Triart C18 ExRS column, delivering high-resolution separation of 11 cannabinoids in less than 6 minutes. Optimised for challenging separations, this column ensures efficiency without compromising accuracy...

YMC Accura columns are the solution for researchers working with challenging phosphate-rich molecules or metal-coordinating compounds. These bioinert columns feature an advanced inert coating, designed to prevent peak tailing and carry-over, while delivering optimal recovery rates. Their seamless compatibility with MS detectors, as demonstrated in this technical note, ensures enhanced workflow accuracy, making them an indispensable tool for high-sensitivity applications...

Maximise efficiency and precision in the analysis of monoclonal antibodies (mAbs) and antibody-drug-conjugates (ADCs) with YMC's tailored solutions. This collection of applications combines proven reliability with cutting-edge technologies to optimise your workflows and save valuable lab time. From intact to fragmented mAbs, DAR determination, or native LC-MS techniques, YMC columns provide highly sensitive and reliable methods using ion exchange (IEX), size exclusion (SEC), hydrophobic interaction (HIC), and reversed-phase liquid chromatography (RP)...

Fast lipidomic analysis with high resolution of the molecular species. Achieve high-resolution lipidomic LC-MS/MS analysis with YMC Accura Triart C8 columns. Ideal for clinical samples, this new method ensures fast comprehensive separation and reliable results from the first injection. Download the detailed Application Note to see the full method and benefits...

The columns provide very sharp peaks with high sensitivities due to an efficient bioinert surface coating of the column body and frits. YMC Accura BioPro IEX columns are the 1st choice for high resolution analysis of oligonucleotides, antibodies, and proteins – especially when coupled with mass spectrometry. Their non-porous hydrophilic polymer beads enable high efficiencies and high throughput analyses. The superior lot-to-lot reproducibility guarantees highly reliable results...

Deepen your knowledge in this exciting workshop designed specifically for biochromatography users. Gain a comprehensive insight into the latest trends and methods in the chromatographic analysis of antibodies, proteins and peptides. You will receive important background information and valuable tips for practical application from experienced workers in biopharmaceutical research and development as well as experienced specialists in (U)HPLC separations and detectors...

An optimised separation of up to 48 carotenoids was successfully achieved using a reliable YMC Carotenoid column with C30 modification. This allowed a mixture of stereomutated carotenoid standards to be detected at different wavelengths. The success of this method was confirmed with a human plasma sample. 24h after the consumption of carotenoid-containing vegetables and fruits, several known carotenoids as well as three unidentified peaks were detected...

This handbook is the perfect companion on your way to trouble-free routine results. Its 40 pages are full of helpful tables, preventative measures and tips to eliminate unwanted effects on your analyses. The (U)HPLC Troubleshooting Guide provides an easy breakdown of possible solutions to the most common problems in analytical liquid chromatography. Take the first step towards a trouble-free routine and get your digital copy of this comprehensive guide...

Artificial sweeteners are added to many foods and cosmetic products. As useful as they are in these areas, they pollute wastewater and groundwater. Therefore, powerful LC-MS/MS applications for the most common artificial sweeteners acesulfame, saccharin and cyclamateare essential. Find out more about how to implement an ultra-fast analysis routine in your lab by downloading the application note...

Chromatographic purification on a research scale is a must-have in many areas such as academia and pharmaceutical research. However, this can be very time consuming. Therefore, effective development of the purification method is essential. These guidelines from YMC demonstrate the main steps for an effective purification method development...

SEC is a suitable method for the analysis of long chain oligonucleotides with length up to 200mer. The choice of the optimal pore size is crucial for the success of the method. In this Application Note, analyses of oligonucleotides of various lengths are described and a selection guide for columns for different DNA is discussed - a practical addition to your local application database...

On January 1st 2023 the 11th edition of the European Pharmacopoeia (Ph. Eur.) took effect. In course of revision, it was harmonised with the United States Pharmacopoeia (USP), British Pharmacopoeia (BP) and Japanese Pharmacopoeia (JP) so that the general chapter 2.2.46 on chromatographic separation techniques received an update. To keep you up to date on the permitted changes, YMC's technical experts have compiled for you these new rules in a helpful technical note, which lists all relevant allowed changes on one page...

This new application note describes the analysis of 7 RNA markers (100 – 1000 bases) using the recently introduced bioinert YMC-Accura Triart Bio C4 column at pH 7 and 80 °C. While conventional column hardware leads to peak tailing and loss of recovery when analysing oligonucleotides, the bioinert coated YMC-Accura Triart Bio C4 column shows excellent recoveries and peak shapes for all 7 well resolved markers...

Molnupiravir (Lagevrio®) is an antiviral medication to treat mild to medium COVID-19 infection. Molnupiravir is actually a prodrug of the active substrate N4-hydroxycytidine (NHC), a synthetic ribonucleoside analogue. The treatment leads to the introduction of copying errors during viral RNA replication and by this inhibits the viral reproduction. This new Application Note demonstrates how molnupiravir and its active agent β-d-N4-hydroxycytidine can be separated by using a YMC-Triart C18 UHPLC column...

New Application note from YMC - Fast UHPLC Analysis of Biomarkers Related to Tobacco Smoke Exposure using YMC-Triart C18 and YMC-Triart Diol-HILIC. Both RP and HILIC allow for high resolution peaks and fast separation. In addition to nicotine, the metabolite cotinine is also determined. The challenge here is that this is only detectable for a short time. NNAL, a breakdown product of the lung carcinogen NNK, is also suitable. In addition, human cells are exposed to oxidative stress when smoking...

Hands-on cannabis testing! The market for cannabis products is expected to continue growing whilst requiring high quality equipment with excellent reproducibility for their complex analysis. In this whitepaper, the common challenges in cannabis testing by liquid chromatography are summarised. Numerous applications show how to select the ideal stationary phase for obtaining high resolution in cannabis potency testing excellent selectivity in pesticide analyses and sharp peak shapes in mycotoxin analyses...

Phosphoramidites are the essential part in the chemical synthesis of oligonucleotides, short fragments of nucleotides and analogues. During the synthesis, errors can occur which is why the phosphoramidites’ purity needs to be closely monitored. In this application note, it is shown how to analyse phosphoramidites using two different separation modes: reversed phase (RP) and normal phase (NP) chromatography. Due to the complexity of the analytes different interactions can be used in order to achieve acceptable retenetion and resolution...

Fast UHPLC Analysis of Cell Penetrating Peptides Using a YMC-Triart C18 column. Cell penetrating peptides (CPPs) are an interesting class of compounds since they are capable of transporting themselves across a cell membrane and also carry so-called “cargo” molecules with them. Due to their advantages over other transport mechanisms they are a focus for current research...

Do you want to know how to choose the right column for your separation? Then check out our new technical note focusing on how to select the perfect LC column! In this technical note, the differences in selectivity and resulting separation behaviour of various phases are shown. Primary and secondary interactions between the analyte and stationary phase are explained for each phase. Two application examples demonstrate how these interactions affect your analysis...

Get to know the significant advantages of YMC-Triart Bio C4! This new application note shows why this stationary phase is the ideal choice for the analysis of cyclic peptides. These compounds are highly valuable drug candidates since their cyclic ring structure provides various advantages over their linear counterpart. This is the reason why they are increasingly becoming the focus in biopharmaceutical research...

YMC-Triart C18 UHPLC columns with an ID of 1 mm are the ideal choice for high sensitivity LC/MS analyses. In this new application note, the screening of 6 antineoplastic drugs is shown. This analysis requires the highest sensitivity since even the smallest amounts of these drugs need to be detected due to their adverse very serious, life-threatening effects. By choosing a high efficiency column with a small internal diameter of 1 mm and coupling it to mass spectrometry the benefit becomes clear: excellent peak shapes – also for low sensitivity compounds!

The use of only one SEC column brings a certain limitation due to its dedicated pore size and a limited column volume. By coupling two columns this hurdle can be overcome. Doubling the column length implies a doubled column volume which results in improved resolution of peaks. Additionally, if using two columns with different pore size, the applicable molecular weight range can be increased significantly....

Are you plagued with poor peak shapes, low sensitivities, sample carry over and/or low recoveries for challenging compounds. Several substances are able to interact with the usual inert column hardware materials such as stainless steel or titanium. The adsorption on metallic surfaces typically leads to peak tailing, loss of recovery and sample carry over. Critical substances are especially metal-coordinating compounds, such as fumonisins, sphingophospholipids or organophosphates in general....

A number of analytes are able to interact with conventional inert column hardware materials such as stainless steel or titanium. The inert YMC-Triart metal-free (U)HPLC columns are fully biocompatible due to their PEEK lined stainless steel column bodies and the use of PEEK frits. You can achieve excellent peak shapes and reliable recoveries for critical substances such as biomolecules. The prevention of carryover makes them an ideal choice for highly sensitive LC/MS analyses....

As the COVID-19 pandemic continues, the worldwide search for an effective treatment is still ongoing. Remdesivir and GS-441524 are antiviral drugs that have been examined for the treatment of COVID-19 caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2). Remdesivir is a nucleotide analogue inhibitor that can inhibit the replication of animal and human coronaviruses...

Method of choice: The combination of size exclusion chromatography (SEC) and light scattering detection (LS) determines molecular weight, especially for higher molecular species such as aggregates or oligomers. As an additional plus sample size and topology can also be revealed. Special attention needs to be turned to the quality of SEC columns in order to provide signals unaffected by spikes. YMC’s SEC columns support LS-detection perfectly due to a high level of inertness...

Have you ever experienced problems with a new method? Are your system suitability tests failing now for a method established years ago? Is is your HPLC column dying too fast? There are many possible reasons for these issues and their root cause can be hard to find. Our new (U)HPLC Troubleshooting Guide provides an easy breakdown of potential solutions for the most common issues in analytical liquid chromatography...

Are you dealing with demanding substances that tend to coordinate with metals? Are you looking for an inert solution for your peptide/protein, (oligo)nucleotide or sensitive small molecules? This is the solution for your problems! The bioinert YMC-Triart columns are fully biocompatible due to their PEEK lined stainless steel column bodies and the use of PEEK frits. You can achieve excellent peak shapes for sensitive compounds. Check out YMC's new flyer to discover more about biocompatible YMC-Triart metal-free columns....

Do you analyse common cannabinoids? This application note shows how you can separate a mixture of 11 of the most common cannabinoids using the robust and reproducible YMC-Triart C18 column. Until recently, cannabis and its derivatives were widely restricted under legislation which stated they had no medical value and carried a substantial risk of misuse. Policy is rapidly changing, and cannabis can now be prescribed for medicinal use in many countries across Europe.

In this application note the drug-to-antibody ratio (DAR) of a cys-linked ADC-mimic is determined using online native HIC-MS. Using the innovative BioPro HIC BF column and a direct coupling to the mass spectrometer even degraded payloads can be detected. The application also demonstrates how to overcome the high salt concentrations which are necessary for HIC methods....

Okadaic acid is diarrhetic toxin (DSP) which can be found in shellfish. Due to its inhibitory effects towards specific protein phosphatases it is a promising compound for various potential medical uses. This application note shows the LC-MS/MS analysis of okadaic acid and its derivates with different YMC-Triart phases. All phases are suitable for the separation of these compounds with high resolution....

In some methods derived from the Pharmacopoeia the stipulation of a specific injection solvent can lead to issues with peak shape and a reduced sensitivity for some or all compounds analysed. With a custom injection program, you can overcome this hurdle and still be conform to Pharmacopoeia guidelines. Your gain will be a higher chance to achieve your system suitability criterions and a more reproducible and robust analysis...

Are you looking for robust and reproducible columns to evaluate peptides? Do you want to separate oligonucleotides with high resolution? Robust YMC-Triart C18 columns are the perfect choice for reliable and reproducible (U)HPLC analyses of peptides and oligonucleotides with exceptional resolution. The wide temperature- (up to 90°C) and pH-range (1-12) provide increased flexibility....

In order to address separation requirements of different compounds, YMC offers two different HIC columns, BioPro HIC HT and BioPro HIC BF. They both have in common their non-porous polymethacrylate particle, while their properties and chromatographic behaviours are quite different. In this application note the retention behaviour of BioPro HIC HT and BioPro HIC BF columns is compared by separating two commercial monoclonal antibodies from each other.

(U)HPLC analysis of original composition of cannabis plant material is necessary for such purposes as phenotype determination and quality control of medical cannabis used for therapeutic treatment. For fast and secure results YMC developed robust (U)HPLC methods for RP and chiral LC. Also, challenging distinction between cannabigerol (CBG) andcannabidiol (CBD) are easily achieved....

If you are looking for a column to separate your antibodies from their fragments as well as from their aggregates, YMC-SEC MAB is your first choice! The column is designed to separate high and low molecular weight ranges, due to its 250 Å pore size. High column stability as well as excellent reproducibility is guaranteed!  The 2019 SCIENTISTS’ CHOICE AWARD NOMINEE is designed for...

Are you working with cannabinoids in medicinal products? This application note describes how to obtain a fast separation of cannabidiol and melatonin which are compounds that can be used in medication for sleep disorders. Excellent peak shapes are achieved using YMC-Triart C18 in less than 7 min. Get your copy of the application note now....

Are you required to analyse very small sample amounts? Or do you need to analyse very low concentrations of antibodies in different kinds of matrices? By using of microLC-MS/MS, only a very small amount of valuable samples, e.g. solutions prepared for patient application (usually a dilution of the dosage form), is required to identify and quantify. In addition, microLC-MS/MS can also be used to monitor residues on surfaces or materials in hospitals, pharmacies or laboratories that prepare solutions for patient application....

You are looking for a state-of-the-art hydrophobic interaction chromatography column? With high resolution at high throughput, but also with superior batch-to-batch reproducibility? Then BioPro HIC HT is the ideal choice for you! YMC’s HIC column, BioPro HIC HT, is especially designed for analysis of biopharmaceuticals such as antibody-drug-conjugates (ADCs). BioPro HIC HT columns are the ideal choice for drug-to-antibody ratio (DAR) determination due to a novel and optimised surface chemistry....

Save time and order prepacked glass columns! Are you lacking the time to pack your column? Or don’t you have sufficient experience? Here is the solution! Rely on the experience of YMC and use prepacked ECO or ECOPLUS columns. Our packing expertise is your advantage. You may choose from the following packing services for ECO and ECOPLUS columns in standard and custom dimensions...

This application note shows a high-resolution UHPLC separation using a robust YMC-Triart C18 column including the previous sample preparation. The simultaneous analysis of etizolam, triazolam, and their metabolites 8-ethylhydroxyetizolam, alpha-hydroxyetizolam, 4-hydroxytriazolam and alpha-hydroxytriazolam in biological samples is performed by MS/MS detection....

Are you working with YMC’s classic columns such as YMC-Pack Pro C18, YMC-Pack ODS-A/AQ or YMC-Pack Polyamine II? Are you looking for specific information such as phase specifications etc for these columns? In that case the new YMC Classics eCatalogue is your perfect resource for this kind of data....

As chromatographers, we all know the problems during method development - “Which phase is suitable and allows a simple and robust separation?” In the field of biochromatography, phase selection is a key to success!

Are you looking for a new ion exchange material?
Have you tried the new BioPro material from YMC - it will give you a significant alternative.

The method transfer from a conventional column to an ultra fast analysis for alkaloids has shown that the use of YMC-UltraHT 2 µm column and adjustment of flow parameters for the smaller column dimensions can reduce the analyses time by about 85% accompany with significant solvent savings and significant solvent savings whilst increasing the sample throughput in the laboratory.

YMC is pleased to introduce Exphere, a new family of bulk packing materials, specifically designed for preparative liquid chromatography. Exphere combines YMC's novel high strength silica with a special polymeric surface modification technique.